Jiao Wei, Sun Jianling, Zhang Xiaofei, An Qi, Fu Longlong, Xu Wenbing, Xie Hongli, Tang Xiuming, Liu Jianxin, Hu Weihong, Gu Yiqun, Zhang Kaishu
Department of Urology, The Affiliated Hospital of Qingdao University, Qingdao, China.
Department of Reproductive Medicine, The Affiliated Hospital of Qingdao University, Qingdao, China.
Andrologia. 2021 May;53(4):e13923. doi: 10.1111/and.13923. Epub 2021 Feb 14.
This study established an oligoasthenospermic rat model using tripterygium glycosides (TGs) and investigated the mechanism by which Qilin pills (QLPs) ameliorate reproductive hypofunction. Thirty-two male Sprague Dawley rats were allocated to four equal-sized groups: (1) the control group received continuous physiological levels of saline; (2) the oligoasthenospermia model group was induced with TGs by daily intragastric administration for 28 days; (3 and 4) oligoasthenospermic rats were treated intragastrically with low dose (1.62 g kg d ) and high dose (3.24 g kg d ) of QLPs once daily for 60 days. The QLP-treated rats showed a marked increase (p < .05) in testicular mass, testicular index and semen parameters compared with the untreated rats. Histopathologically, the QLP-treated groups exhibited restored seminiferous tubules in contrast to the model group. Reactive oxygen species and malondialdehyde levels were dramatically decreased (p < .05) in the testes of the QLP-treated rats. QLP treatment partly reverted (p < .05) the circulatory levels of reproductive hormones (FSH, LH, testosterone, prolactin and SHBG) and hepatic and renal function (AST, Cr and urea). Our results showed that oral QLP treatment had a curative effect on the testicular mass, sperm quality, testicular pathomorphology, antioxidants, plasmatic hormones, and liver and renal function of rats.
本研究利用雷公藤多苷（TGs）建立了少弱精子症大鼠模型，并探讨了麒麟丸（QLPs）改善生殖功能低下的机制。将32只雄性Sprague Dawley大鼠分为四组，每组数量相等：（1）对照组给予持续生理水平的生理盐水；（2）少弱精子症模型组通过每日灌胃给予TGs诱导28天；（3和4）少弱精子症大鼠分别以低剂量（1.62 g·kg⁻¹·d⁻¹）和高剂量（3.24 g·kg⁻¹·d⁻¹）的QLPs每日灌胃一次，持续60天。与未治疗的大鼠相比，接受QLP治疗的大鼠睾丸质量、睾丸指数和精液参数显著增加（p < 0.05）。组织病理学检查显示，与模型组相比，接受QLP治疗的组曲细精管得以恢复。接受QLP治疗的大鼠睾丸中的活性氧和丙二醛水平显著降低（p < 0.05）。QLP治疗部分逆转了（p < 0.05）生殖激素（促卵泡激素、促黄体生成素、睾酮、催乳素和性激素结合球蛋白）以及肝肾功能（谷草转氨酶、肌酐和尿素）的循环水平。我们的结果表明，口服QLP治疗对大鼠的睾丸质量、精子质量、睾丸病理形态、抗氧化剂、血浆激素以及肝肾功能具有治疗作用。
