Hebei Key Laboratory of Reproductive Medicine, Hebei Institute of Reproductive Health, Hebei Reproductive Health Hospital, Shijiazhuang, 050071, China.
Graduate School of Hebei Medical University, Shijiazhuang, 050017, China.
Chin J Integr Med. 2024 Apr;30(4):330-338. doi: 10.1007/s11655-023-3750-9. Epub 2024 Jan 12.
To determine the possible protective effects of Jinghuosu, a dietary supplement (DS), on tripterygium glycosides (TG)-induced reproductive system injury in rats and its underlying mechanisms.
A reproductive damage model was established in rats by feeding of TGs. Twenty-eight male Sprague Dawley rats were randomly divided into 4 groups using a random number table (n=7 in each): control (C) group, model (M) group, DS group and L-carnitine (LC) group. Rats in M, DS and LC groups received 40 mg/kg TGs orally. Starting from the 5th week, after administration of TGs for 4 h every day, rats in DS and LC groups were administered with 2.7 g/kg DS and 0.21 g/kg LC, respectively, for protective treatment over the next 4 weeks. Rats in Group C continued to receive the control treatment. Hematoxylin-eosin staining was used for histopathological analysis of rat testicular tissues. Enzyme-linked immunosorbent assay was performed to measure alkaline phosphatase (ALP), lactate dehydrogenase, alcohol dehydrogenase, total antioxidant capacity (T-AOC), superoxide dismutase, glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) concentrations. Chemiluminescence assay was used to determine the serum testosterone content. Quantitative real-time PCR and Western blotting were conducted to analyze the expression of genes and proteins related to the testosterone synthesis pathway and the nuclear factor erythroid 2-related factor 2/heme oxygenase 1 antioxidant pathway.
Oral administration of TGs induced significant increases in the testicular levels of zinc transporter 1 and MDA (P<0.05). On the other hand, sperm concentration, sperm motility, and serum testosterone, serum zinc, testicular zinc, Zrt-, Irt-like protein 1, ALP, luteinizing hormone (LH) receptor, steroidogenic acute regulatory protein, Cytochrome P450 family 11 subfamily A member 1, 3 β -hydroxysteroid dehydrogenase 1 T-AOC, GSH-Px, nuclear factor erythroid 2-related factor 2, heme oxygenase-1 and NAD (P)H: quinone oxidoreductase 1 levels decreased following TGs exposure (P<0.05). All of these phenotypes were evidently reversed by DS (P<0.05).
DS Jinghuosu protects against TG-induced reproductive system injury in rats, probably by improving zinc homeostasis, enhancing the testosterone synthesis and attenuating oxidative stress.
探讨膳食补充剂荆花素对雷公藤苷致大鼠生殖系统损伤的可能保护作用及其机制。
采用雷公藤苷灌胃建立大鼠生殖损伤模型。28 只雄性 Sprague Dawley 大鼠采用随机数字表法分为 4 组（n=7）：对照组（C 组）、模型组（M 组）、荆花素组（DS 组）和左旋肉碱（LC）组。M 组、DS 组和 LC 组大鼠每日灌胃给予 40mg/kg 雷公藤苷，连续 4 周。自第 5 周起，每天灌胃 4h 后，DS 组和 LC 组大鼠分别给予 2.7g/kg 荆花素和 0.21g/kg 左旋肉碱进行保护性治疗，共 4 周。C 组大鼠继续给予对照处理。采用苏木精-伊红染色法观察大鼠睾丸组织的病理变化，酶联免疫吸附法检测碱性磷酸酶（ALP）、乳酸脱氢酶、醇脱氢酶、总抗氧化能力（T-AOC）、超氧化物歧化酶、谷胱甘肽过氧化物酶（GSH-Px）和丙二醛（MDA）浓度，化学发光法检测血清睾酮含量，实时荧光定量 PCR 和 Western blot 法检测与睾酮合成途径和核因子红细胞 2 相关因子 2/血红素加氧酶 1 抗氧化途径相关的基因和蛋白表达。
雷公藤苷灌胃导致大鼠睾丸锌转运体 1 和 MDA 水平显著升高（P<0.05）。另一方面，精子浓度、精子活力和血清睾酮、血清锌、睾丸锌、Zrt-、Irt 样蛋白 1、ALP、促黄体生成素（LH）受体、类固醇急性调节蛋白、细胞色素 P450 家族 11 亚家族 A 成员 1、3β-羟甾脱氢酶 1、T-AOC、GSH-Px、核因子红细胞 2 相关因子 2、血红素加氧酶-1 和 NAD（P）H：醌氧化还原酶 1 水平在雷公藤苷暴露后降低（P<0.05）。所有这些表型均被 DS 明显逆转（P<0.05）。
荆花素可能通过改善锌稳态、增强睾酮合成和减轻氧化应激来防治雷公藤苷所致的生殖系统损伤。
