Sweeney J D, Labuzetta J W, Fitzpatrick J E
Hemophilia Center of Western New York, Roswell Park Memorial Institute, Buffalo, New York 14263.
Am J Clin Pathol. 1988 May;89(5):655-9. doi: 10.1093/ajcp/89.5.655.
Platelet aggregation studies are usually performed in either electrical impedance or optical systems and the release reaction assessed by quantitating adenosine triphosphate (ATP) from luminesence produced by the firefly luciferin-luciferase system. In evaluating the results of such studies, attention is paid to a variety of parameters such as the slope of the aggregation response and the maximum aggregation expressed as percent light transmission in optical and as Ohms in electrical impedance systems. Although threshold platelet counts are frequently cited below which the performance of these studies is technically difficult, the influence of the platelet count within the normal range on the results of such studies has not been prospectively addressed. This study examines the relationship between the aggregation response, ATP release and the platelet count in a lumi-impedance system. It is clear that the platelet count influences the results in this system and requires consideration in the interpretation if an erroneous conclusion is to be avoided.
血小板聚集研究通常在电阻抗或光学系统中进行，释放反应通过萤火虫荧光素 - 荧光素酶系统产生的发光来定量三磷酸腺苷（ATP）进行评估。在评估此类研究结果时，会关注各种参数，如聚集反应的斜率以及在光学系统中以透光百分比表示、在电阻抗系统中以欧姆表示的最大聚集率。尽管经常提到血小板计数阈值，低于该阈值进行这些研究在技术上具有难度，但正常范围内血小板计数对此类研究结果的影响尚未得到前瞻性探讨。本研究在光 - 阻抗系统中考察了聚集反应、ATP释放与血小板计数之间的关系。很明显，血小板计数会影响该系统的结果，若要避免得出错误结论，在解释结果时需加以考虑。
