Department of Pharmacognosy, Baqai Institute of Pharmaceutical Sciences, Baqai Medical University, Karachi, Pakistan.
Department of Pharmacognosy, Faculty of Pharmacy, Dow University of Health Sciences, Karachi, Pakistan.
Pak J Pharm Sci. 2020 Jul;33(4):1641-1657.
The object of this study is to investigate the quality of various plant materials used in the preparation of herbal formulations using different methods of standardization to confirm their purity, safety and efficacy. However, it is uncertain whether these raw materials comply with the standards prescribed in the pharmacopeias. In the present study six raw materials' i.e. Foeniculum vulgarae, Curcuma longa, Aloe barbadensis, Plantago ovata, Zingiber officinale and Glycyrrhiza glabra have been obtained from the market and various quality control tests including microscopic evaluation, physico-chemical characteristics, thin layer chromatography (TLC), spectrophotometric assay (British Pharmacopoeia) and Fourier transform infrared spectroscopy (FTIR) have been performed to determine their compliance with the standards. The TLC has been used for the identification of the active ingredients on comparison of their R values with the reference standard. FTIR Spectra of these materials have been obtained to assign the functional groups present in the components of a particular material. Although these findings provide a significant data to herbal drug manufacturers for authentication of commercially available plant materials used in various herbal formulation.
本研究的目的是通过使用不同的标准化方法来研究各种用于草药制剂的植物材料的质量,以确认其纯度、安全性和功效。然而,这些原材料是否符合药典规定的标准尚不确定。在本研究中,从市场上获得了六种原材料,即茴香、姜黄、巴巴多斯芦荟、车前草、生姜和甘草,并进行了各种质量控制测试,包括显微镜评估、物理化学特性、薄层色谱 (TLC)、分光光度法测定(英国药典)和傅里叶变换红外光谱 (FTIR),以确定它们是否符合标准。TLC 已用于通过比较其 R 值与参考标准来鉴定活性成分。对这些材料的 FTIR 光谱进行了分析,以确定特定材料成分中存在的官能团。尽管这些发现为草药药物制造商提供了重要数据,用于鉴定各种草药配方中使用的市售植物材料。