Tripathi Leena, Ntui Valentine Otang, Tripathi Jaindra Nath, Kumar P Lava
International Institute of Tropical Agriculture, Nairobi, Kenya.
International Institute of Tropical Agriculture, Ibadan, Nigeria.
Front Microbiol. 2021 Jan 27;11:609784. doi: 10.3389/fmicb.2020.609784. eCollection 2020.
Viral diseases are significant biotic constraints for banana ( spp.) production as they affect the yield and limit the international movement of germplasm. Among all the viruses known to infect banana, the banana bunchy top virus and banana streak viruses are widespread and economically damaging. The use of virus-resistant bananas is the most cost-effective option to minimize the negative impacts of viral-diseases on banana production. CRISPR/Cas-based genome editing is emerging as the most powerful tool for developing virus-resistant crop varieties in several crops, including the banana. The availability of a vigorous genetic transformation and regeneration system and a well-annotated whole-genome sequence of banana makes it a compelling candidate for genome editing. A robust CRISPR/Cas9-based genome editing of the banana has recently been established, which can be applied in developing disease-resistant varieties. Recently, the CRISPR system was exploited to detect target gene sequences using Cas9, Cas12, Cas13, and Cas14 enzymes, thereby unveiling the use of this technology for virus diagnosis. This article presents a synopsis of recent advancements and perspectives on the application of CRISPR/Cas-based genome editing for diagnosing and developing resistance against banana viruses and challenges in genome-editing of banana.
病毒病是香蕉（品种）生产中的重要生物限制因素，因为它们会影响产量并限制种质的国际流动。在所有已知感染香蕉的病毒中，香蕉束顶病毒和香蕉条纹病毒分布广泛且具有经济破坏性。使用抗病毒香蕉是将病毒病对香蕉生产的负面影响降至最低的最具成本效益的选择。基于CRISPR/Cas的基因组编辑正在成为包括香蕉在内的几种作物中培育抗病毒作物品种的最强大工具。香蕉拥有强大的遗传转化和再生系统以及注释完善的全基因组序列，这使其成为基因组编辑的有力候选对象。最近已建立了基于CRISPR/Cas9的强大香蕉基因组编辑方法，可用于培育抗病品种。最近，利用CRISPR系统通过Cas9、Cas12、Cas13和Cas14酶检测目标基因序列，从而揭示了该技术在病毒诊断中的应用。本文概述了基于CRISPR/Cas的基因组编辑在香蕉病毒诊断和抗性培育方面的最新进展和前景，以及香蕉基因组编辑面临的挑战。
