Nikzamir Abdolrahim, Rezaei-Tavirani Mostafa, Razzaghi Mohhamadreza, Rezaei Tavirani Sina, Hamzeloo-Moghadam Maryam, Esmaeili Somayeh, Hatami Behzad, Ahmadzadeh Alireza
Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Gastroenterol Hepatol Bed Bench. 2020 Winter;13(Suppl1):S98-S105.
Evaluation of deregulated genes after long-term consuming of omeprazole via network analysis.
Proton pump inhibitors (PPIs) are used to inhibit gastric high rate of acid secretion in patients. Omeprazole as a PPI is a common drug in this regard. Evaluation of long-term consumption of omeprazole is studied in the present study via its effects on the gene expression of "human coronary artery endothelial cells".
Net effect of the presence of omeprazole on gene expression profiles of "human coronary artery endothelial cells" was evaluated through data from gene expression omnibus (GEO). Results of protein-protein interaction (PPI) network analysis were assessed via biological process examination to find the critical deregulated genes after long-term consumption of omeprazole.
"Negative regulation of muscle cell apoptotic process", "negative regulation of DNA binding", "telencephalon cell migration", "forebrain cell migration" "response to cadmium ion", "cell-cell recognition", "positive regulation of protein targeting to mitochondrion", and "central nervous system neuron development" were the clusters of biological processes that were associated to the long -term presence of omeprazole. The final critical deregulated genes were JAK2, PTK2, and NRG1.
It can be concluded that cell cycle, proliferation, and apoptosis and several essential biological processes are affected and nervous system is a possible target related to the long-term consumption of omeprazole.
通过网络分析评估长期服用奥美拉唑后失调的基因。
质子泵抑制剂（PPIs）用于抑制患者胃内的高胃酸分泌率。奥美拉唑作为一种质子泵抑制剂，是这方面常用的药物。本研究通过奥美拉唑对“人冠状动脉内皮细胞”基因表达的影响，来研究其长期服用情况。
通过基因表达综合数据库（GEO）的数据，评估奥美拉唑对“人冠状动脉内皮细胞”基因表达谱的净效应。通过生物学过程检查评估蛋白质-蛋白质相互作用（PPI）网络分析的结果，以找出长期服用奥美拉唑后关键的失调基因。
“肌肉细胞凋亡过程的负调控”“DNA结合的负调控”“端脑细胞迁移”“前脑细胞迁移”“对镉离子的反应”“细胞-细胞识别”“蛋白质靶向线粒体的正调控”以及“中枢神经系统神经元发育”是与长期存在奥美拉唑相关的生物学过程簇。最终关键的失调基因是JAK2、PTK2和NRG1。
可以得出结论，细胞周期、增殖和凋亡以及几个重要的生物学过程会受到影响，并且神经系统可能是与长期服用奥美拉唑相关的一个靶点。
