Shams Saeed, Mousavi Nasab Seyed Dawood, Heydari Hosein, Tafaroji Javad, Ahmadi Nayebali, Shams Afzali Esmaeil
Cellular and Molecular Research Center, Qom University of Medical Sciences. Qom, Iran.
Department of Research and Development, Pasteur Institute of Iran, Tehran, Iran.
Gastroenterol Hepatol Bed Bench. 2020 Winter;13(Suppl1):S128-S133.
The aim of the study is to estimate the burden of Rotavirus gastroenteritis as well as predominant genotypes of Rotavirus among children less than 5 years of age referring to Pediatric University Hospital in Qom, Iran.
Gastroenteritis is the fourth most common cause of death and accounts for 16% of all deaths in children <5 years of age worldwide.
During two years, 130 patients referring to a pediatric hospital were enrolled in this study. After RNA extraction, Rotaviruses were detected by the VP6 gene. Then, G-typing (G1, G2, G3, G4, G8, G9, and G12) and P-typing (P4, P6, and P8) were performed using RT-PCR and specific primers.
The results of the PCR revealed that from a total of 130 patients, 22 cases (16.9%) showed positive VP6 by RT-PCR. G1 was mostly the predominant serotype (27%), accounting for 22% of all VP7-positive isolates, followed by G9 (18%), G2 (9%), G3 (9%), and G4 (9%). None of the strains revealed the presence of G8 genotype (0%), and 5 specimens (23%) were non-typable. The frequency of P typing was P8 (50%), P6 (23%), P4 (14%), and 3 samples were P-non-typable (13%), respectively. The dominant G-P combination was G1 [8] (32%).
Such studies based on typing methods assists in the Rotavirus vaccine introduction by policymakers and design of new effective vaccines.
本研究旨在评估伊朗库姆市儿科大学医院5岁以下儿童轮状病毒肠胃炎的负担以及轮状病毒的主要基因型。
肠胃炎是全球5岁以下儿童第四大常见死因，占所有死亡人数的16%。
在两年时间里，本研究纳入了130名到儿科医院就诊的患者。提取RNA后，通过VP6基因检测轮状病毒。然后，使用RT-PCR和特异性引物进行G分型（G1、G2、G3、G4、G8、G9和G12）和P分型（P4、P6和P8）。
PCR结果显示，在总共130名患者中，22例（16.9%）通过RT-PCR检测出VP6呈阳性。G1大多是主要血清型（27%），占所有VP7阳性分离株的22%；其次是G9（18%）、G2（9%）、G3（9%）和G4（9%）。没有菌株显示存在G8基因型（0%），5个样本（23%）无法分型。P分型的频率分别为P8（50%）、P6（23%）、P4（14%），3个样本无法进行P分型（13%）。主要的G-P组合是G1[8]（32%）。
此类基于分型方法的研究有助于政策制定者引入轮状病毒疫苗，并设计新的有效疫苗。
