Hatchell D L, McAdoo T, Sheta S, King R T, Bartolomé J V
Department of Ophthalmology, Duke University, Durham, NC.
Arch Ophthalmol. 1988 May;106(5):669-72. doi: 10.1001/archopht.1988.01060130731033.
Proliferation of host cells from around the optic nerve head has recently been implicated in the development of experimental proliferative vitreoretinopathy in rabbit eyes injected with homologous fibroblasts. We used liquid scintillation spectrometry to quantitate the tritiated thymidine incorporation into cells in the vitreous, retina, and optic nerve head following intravitreal injection of 250,000 homologous dermal fibroblasts. Cellular proliferation peaked three days after injection of the fibroblasts. The amount of tritiated thymidine incorporation that occurred three days following injection of irradiated homologous fibroblasts (incapable of cellular division) was not significantly different than that following injection of normal homologous fibroblasts, indicating that host cells were responsible for most of the cellular proliferation. Treatment with fluorouracil or triamcinolone acetonide completely arrested cellular proliferation following injection of normal fibroblasts.
最近发现，在向兔眼注射同源成纤维细胞后诱发的实验性增殖性玻璃体视网膜病变的发病过程中，视神经乳头周围的宿主细胞出现了增殖。我们运用液体闪烁光谱法，对玻璃体内、视网膜以及视神经乳头的细胞在玻璃体内注射250,000个同源真皮成纤维细胞后掺入氚标记胸腺嘧啶核苷的情况进行了定量分析。细胞增殖在注射成纤维细胞三天后达到峰值。注射经辐照的同源成纤维细胞（无法进行细胞分裂）三天后掺入的氚标记胸腺嘧啶核苷量，与注射正常同源成纤维细胞后的情况相比，没有显著差异，这表明大部分细胞增殖是由宿主细胞引起的。在注射正常成纤维细胞后，用氟尿嘧啶或曲安奈德进行治疗可完全抑制细胞增殖。
