Handa J T, Berkowitz B A, Wilson C A, Ando N, Sen H A, Jaffe G J
Duke Eye Center, Durham, North Carolina, USA.
Graefes Arch Clin Exp Ophthalmol. 1996 Jan;234(1):43-6. doi: 10.1007/BF00186517.
Although the mechanism of preretinal neovascular growth in the cell-injected rabbit eye model is not known, it has been proposed that the initial vasodilation and eventual development of neovascularization may be attributable to inflammatory mediators. However, an alternative explanation involving hypoxia has not been considered. The purpose of this study was to measure preretinal oxygen tension prior to the development of preretinal neovascularization in the cell-injected rabbit eye.
In the rabbit, intravitreous injections of 250,000 homologous dermal fibroblasts were performed on one eye; the fellow (control) eye was injected with vehicle. Preretinal oxygen tension over the myelin wing was measured using 19F-NMR spectroscopy of a 30-microliters droplet of perfluorocarbon previously injected into the preretinal vitreous.
Compared to control eyes, fibroblast-injected eyes showed a 1.7-fold decrease in preretinal oxygen tension from the first time studied (1 day after cell injection) through the development of visible neovascularization. Hypoxia occurred without coexisting ophthalmoscopic evidence of vascular occlusion or, on days 1 and 3 after cell injection, retinal detachment.
This result demonstrates for the first time that preretinal hypoxia precedes the development of preretinal neovascularization in the fibroblast-injected rabbit eye.
尽管细胞注射兔眼模型中视网膜前新生血管生长的机制尚不清楚，但有人提出，最初的血管舒张和最终的新生血管形成可能归因于炎症介质。然而，尚未考虑涉及缺氧的另一种解释。本研究的目的是在细胞注射兔眼中视网膜前新生血管形成之前测量视网膜前氧张力。
在兔的一只眼中玻璃体内注射250,000个同源真皮成纤维细胞；对侧（对照）眼注射赋形剂。使用先前注射到视网膜前玻璃体中的30微升全氟碳液滴的19F-NMR光谱测量髓鞘翼上的视网膜前氧张力。
与对照眼相比，从首次研究（细胞注射后1天）到可见新生血管形成，注射成纤维细胞的眼睛视网膜前氧张力降低了1.7倍。缺氧发生时，没有同时存在眼底镜检查显示的血管阻塞证据，也没有在细胞注射后第1天和第3天出现视网膜脱离。
该结果首次证明，在注射成纤维细胞的兔眼中，视网膜前缺氧先于视网膜前新生血管形成。
