Department of Materials, Department of Bioengineering and Institute of Biomedical Engineering, Imperial College London, Exhibition Road, London SW7 2AZ, United Kingdom.
State Key Laboratory of Chemical Resource Engineering, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China.
ACS Nano. 2021 Mar 23;15(3):4034-4044. doi: 10.1021/acsnano.0c05423. Epub 2021 Feb 15.
The evolution of life on earth eventually leads to the emergence of species with increased complexity and diversity. Similarly, evolutionary chemical space exploration in the laboratory is a key step to pursue the structural and functional diversity of supramolecular systems. Here, we present a powerful tool that enables rapid peptide diversification and employ it to expand the chemical space for supramolecular functions. Central to this strategy is the exploitation of palladium-catalyzed Suzuki-Miyaura cross-coupling reactions to direct combinatorial synthesis of peptide arrays in microtiter plates under an open atmosphere. Taking advantage of this library design, our results unambiguously deliver a fertile platform for creating a set of intriguing peptide functions including green fluorescent protein-like peptide emitters with chemically encoded emission colors, hierarchical self-assembly into nano-objects, and macroscopic hydrogels. This work also offers opportunities for quickly surveying the diversified peptide arrays and thereby identifying the structural factors that modulate peptide properties.
地球上生命的进化最终导致了具有更高复杂性和多样性的物种的出现。同样，实验室中进化化学空间的探索是追求超分子系统结构和功能多样性的关键步骤。在这里，我们提出了一种强大的工具，可以实现肽的快速多样化，并利用它来扩展超分子功能的化学空间。该策略的核心是利用钯催化的 Suzuki-Miyaura 交叉偶联反应，在开放气氛下直接在微量滴定板中进行肽阵列的组合合成。利用这个文库设计，我们的结果明确地提供了一个富有成效的平台，用于创建一系列有趣的肽功能，包括具有化学编码发射颜色的类绿色荧光蛋白肽发射器、分层自组装成纳米物体和宏观水凝胶。这项工作还为快速调查多样化的肽阵列提供了机会，从而确定调节肽性质的结构因素。
