Grasslands Research Centre, AgResearch Limited, Palmerston North, New Zealand.
Invermay Research Centre, AgResearch Limited, Mosgiel, New Zealand.
Sci Rep. 2021 Feb 15;11(1):3836. doi: 10.1038/s41598-021-82815-y.
Anthelmintic treatment of adult ewes is widely practiced to remove parasite burdens in the expectation of increased ruminant productivity. However, the broad activity spectra of many anthelmintic compounds raises the possibility of impacts on the rumen microbiota. To investigate this, 300 grazing ewes were allocated to treatment groups that included a 100-day controlled release capsule (CRC) containing albendazole and abamectin, a long-acting moxidectin injection (LAI), and a non-treated control group (CON). Rumen bacterial, archaeal and protozoal communities at day 0 were analysed to identify 36 sheep per treatment with similar starting compositions. Microbiota profiles, including those for the rumen fungi, were then generated for the selected sheep at days 0, 35 and 77. The CRC treatment significantly impacted the archaeal community, and was associated with increased relative abundances of Methanobrevibacter ruminantium, Methanosphaera sp. ISO3-F5, and Methanomassiliicoccaceae Group 12 sp. ISO4-H5 compared to the control group. In contrast, the LAI treatment increased the relative abundances of members of the Veillonellaceae and resulted in minor changes to the bacterial and fungal communities by day 77. Overall, the anthelmintic treatments resulted in few, but highly significant, changes to the rumen microbiota composition.
对成年绵羊进行驱虫治疗以消除寄生虫负担，从而提高反刍动物的生产力，这种做法在畜牧业中十分普遍。然而，许多驱虫化合物广谱的活性范围使得它们对瘤胃微生物群产生影响的可能性增加。为了研究这一问题，将 300 只放牧绵羊分配到治疗组，包括含有阿苯达唑和阿维菌素的 100 天控释胶囊 (CRC)、长效莫昔克丁注射剂 (LAI) 和未经处理的对照组 (CON)。在第 0 天分析瘤胃细菌、古菌和原生动物群落，以确定每组 36 只具有相似起始组成的绵羊。然后，在第 0、35 和 77 天对选定的绵羊进行微生物组分析，包括瘤胃真菌的微生物组。CRC 处理显著影响了古菌群落，并与对照相比，瘤胃甲烷短杆菌、Methanosphaera sp. ISO3-F5 和 Methanomassiliicoccaceae Group 12 sp. ISO4-H5 的相对丰度增加有关。相比之下，LAI 处理增加了韦荣球菌科的相对丰度，到第 77 天，细菌和真菌群落也发生了较小的变化。总体而言，驱虫处理对瘤胃微生物群组成的影响很小，但具有高度显著性。
