Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, Gifu, Japan.
Ichimaru Pharcos Co., Ltd., Gifu, Japan.
Biosci Biotechnol Biochem. 2021 Feb 24;85(3):493-501. doi: 10.1093/bbb/zbaa071.
The Asian traditional medicinal plant Acorus calamus and its component α-asarone exhibited various biological activities, such as antiinflammation and antioxidant effects. In the present study, we investigated the in vitro effects of A. calamus extract and α-asarone on oxidative stress- and endoplasmic reticulum (ER) stress-induced cell death in hippocampal HT22 cells. A. calamus extract and α-asarone both significantly suppressed cell death induced by the oxidative stress inducer l-glutamate and ER stress inducer tunicamycin. A. calamus extract and α-asarone also significantly reduced reactive oxygen species (ROS) production induced by l-glutamate. Moreover, A. calamus extract and α-asarone suppressed the phosphorylation of protein kinase RNA-like ER kinase (PERK) induced by tunicamycin. These results suggest that A. calamus extract and α-asarone protect hippocampal cells from oxidative stress and ER stress by decreasing ROS production and suppressing PERK signaling, respectively. α-Asarone has potential as a potent therapeutic candidate for neurodegenerative diseases, including Alzheimer's disease.
亚洲传统药用植物菖蒲及其成分α-细辛脑具有多种生物活性，如抗炎和抗氧化作用。在本研究中，我们研究了菖蒲提取物和α-细辛脑对海马 HT22 细胞氧化应激和内质网（ER）应激诱导细胞死亡的体外作用。菖蒲提取物和α-细辛脑均能显著抑制氧化应激诱导剂 l-谷氨酸和 ER 应激诱导剂衣霉素诱导的细胞死亡。菖蒲提取物和α-细辛脑还能显著减少 l-谷氨酸诱导的活性氧（ROS）的产生。此外，菖蒲提取物和α-细辛脑抑制了衣霉素诱导的蛋白激酶 RNA 样内质网激酶（PERK）的磷酸化。这些结果表明，菖蒲提取物和α-细辛脑通过降低 ROS 的产生和抑制 PERK 信号通路分别保护海马细胞免受氧化应激和 ER 应激的影响。α-细辛脑可能是阿尔茨海默病等神经退行性疾病的有效治疗候选药物。
