School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India.
Fitoterapia. 2011 Apr;82(3):369-74. doi: 10.1016/j.fitote.2010.11.009. Epub 2010 Nov 6.
The present study was aimed to investigate the possible interaction of the standardized extract of Acorus calamus (AC) with Cytochrome P450 enzyme, quantitative determination of the α-asarone in the AC rhizome was performed by RP-HPLC method. In vitro interaction of the plant extract was evaluated by CYP450-carbon monoxide complex (CYP450-CO) assay. Effect on individual isoforms such as CYP3A4 and CYP2D6 isozymes were analyzed through fluorescence product formation and respective IC(50) values were determined. CYP450-CO assay showed moderate interaction potential. Extract showed higher IC(50) values (46.84±1.83-32.99±2.21 μg/ml) comparing to the standard inhibitors and lower IC(50) value than α-asarone (65.16±2.37-42.15±2.45 μg/ml).
本研究旨在探讨菖蒲标准化提取物（AC）与细胞色素 P450 酶可能的相互作用，采用反相高效液相色谱法（RP-HPLC）对菖蒲根茎中的α-细辛脑进行定量测定。通过 CYP450-一氧化碳复合物（CYP450-CO）测定法评估植物提取物的体外相互作用。通过荧光产物形成分析对个体同工酶（如 CYP3A4 和 CYP2D6 同工酶）的影响，并测定各自的 IC（50）值。CYP450-CO 测定法显示出中等相互作用潜力。与标准抑制剂相比，提取物的 IC（50）值（46.84±1.83-32.99±2.21 μg/ml）较高，而与 α-细辛脑（65.16±2.37-42.15±2.45 μg/ml）相比，IC（50）值较低。
