Domínguez Gonzalo, Cardiel Eladio, Sánchez Elsa, Hernández Pablo-Rogelio
Department of Electrical Engineering, Bioelectronics section, Center for Research and Advanced Studies of the National Polytechnic Institute, Mexico City, Mexico.
Department of Physiology, Biophysics, and Neuroscience, Center for Research and Advanced Studies of the National Polytechnic Institute, Mexico City, Mexico.
J Radiat Res. 2021 Mar 10;62(2):259-268. doi: 10.1093/jrr/rrab001.
To assess the effects of exposure to extremely low-frequency magnetic fields (ELF-MFs) on MDCK cell lines, experiments were performed in a chamber under controlled conditions (temperature, humidity and CO2). Therefore, the measured physicochemical and electrical changes in the cells are due solely to the magnetic field exposure and not to external factors. A developed sinusoidal magnetic field generator produced the ELF-MFs with a uniform magnetic field and adjustable intensity and frequency. Three experimental indicators were used: (i) transepithelial electrical impedance (TEEI); (ii) cell migration and proliferation; and (iii) expression of the proteins of the tight junctions, and changes in the area and shape of the cell nuclei. No significant effects on TEEI values were observed when 10 and 50 G 60 Hz magnetic fields were applied to confluent cell monolayers. There were no significant differences in migration and proliferation of the cell monolayer exposed to 60 Hz magnetic fields10 and 50 G , but a contact inhibition factor was observed. The expression of the CLDN-1 protein decreased by 90% compared with the control, while ZO-1 protein expression increased by 120%. No significant effects were observed in the area and shape of the cell nuclei. Experimentation in a controlled environment, under physiological conditions, ensures that the observed effects were strictly due to exposure to magnetic fields. Different exposure conditions are necessary to determine the impact on TEEI and cell migration-proliferation indicators.
为了评估暴露于极低频磁场（ELF-MFs）对MDCK细胞系的影响，实验在可控条件（温度、湿度和二氧化碳）的实验室内进行。因此，所测得的细胞理化和电学变化仅归因于磁场暴露，而非外部因素。一台研发的正弦磁场发生器产生了具有均匀磁场、强度和频率可调的极低频磁场。使用了三个实验指标：（i）跨上皮电阻抗（TEEI）；（ii）细胞迁移和增殖；以及（iii）紧密连接蛋白的表达，和细胞核面积与形状的变化。当对汇合的细胞单层施加10 G和50 G 60 Hz磁场时，未观察到对TEEI值有显著影响。暴露于60 Hz 10 G和50 G磁场的细胞单层在迁移和增殖方面没有显著差异，但观察到了接触抑制因子。CLDN-1蛋白的表达与对照组相比下降了90%，而ZO-1蛋白表达增加了120%。在细胞核面积和形状方面未观察到显著影响。在生理条件下的可控环境中进行实验，确保了所观察到的效应严格归因于磁场暴露。需要不同的暴露条件来确定对TEEI和细胞迁移-增殖指标的影响。
