Johnston N E, Estrella R A, Oxender W D
Am J Vet Res. 1977 Sep;38(9):1323-6.
Twenty Holstein-Friesian male calves were obtained within 4 hours after bith (colostrum deprived) and allotted to 1 of 4 groups, each given a different feeding: colostrum, milk replacer, polyvinylpyrrolidone (PVP), and saline solution (0.85% NaCl). Each calf was fed 2 L of the respective diets every 12 hours. Rectal temperatures were recorded and blood samples were collected immediately before each feeding. At approximately 27 hours of age, all calves were inoculated orally with 1.5 X 10(10) viable organisms of a septicemia-producing Escherichia coli serotype O26: K60:NM. Within 8 hours, all calves had diarrhea. Coli-septicemia (E coli cultured from liver, spleen, and cardiac blood) was present in 1 of the 5 calves fed colostrum, in 5 or the 5 calves fed milk replacer, in 5 of the 5 calves fed PVP, and in 4 of the 5 calves fed saline solution. At necropsy of the calves (12 to 48 hours after oral inoculation), the same organism was isolated by cultural technique from small intestines of 19 of the 20 calves. Serum immunoglobulin G concentrations increased (P less than 0.01) in calves fed the colostrum diet in sharp contrast to the agammaglobulinemia occurring in calves fed the milk replacer, PVP, or saline solution. Results indicate that colostrum fed to the calf soon after birth provides protection from colisepticemia, but does not prevent the diarrhea of colibacillosis.
20头荷斯坦-弗里生雄性犊牛在出生后4小时内获取（未喂初乳），并分配到4组中的1组，每组给予不同的饲料：初乳、代乳品、聚乙烯吡咯烷酮（PVP）和盐溶液（0.85%氯化钠）。每头犊牛每12小时喂2升相应的日粮。记录直肠温度，并在每次喂食前立即采集血样。在大约27小时龄时，所有犊牛口服接种1.5×10¹⁰个产败血症的大肠杆菌血清型O26:K60:NM的活菌。8小时内，所有犊牛都出现腹泻。在喂初乳的5头犊牛中有1头出现大肠杆菌败血症（从肝脏、脾脏和心血中培养出大肠杆菌），在喂代乳品的5头犊牛中有5头出现，在喂PVP的5头犊牛中有5头出现，在喂盐溶液的5头犊牛中有4头出现。在犊牛尸检时（口服接种后12至48小时），通过培养技术从20头犊牛中的19头的小肠中分离出相同的细菌。与喂代乳品、PVP或盐溶液的犊牛中出现的无丙种球蛋白血症形成鲜明对比的是，喂初乳日粮的犊牛血清免疫球蛋白G浓度升高（P<0.01）。结果表明，出生后不久给犊牛喂初乳可提供预防大肠杆菌败血症的保护，但不能预防大肠杆菌病的腹泻。
