Alexander N, Kogosov E, Haun C K
Department of Medicine, University of Southern California School of Medicine, Los Angeles 90033.
Brain Res. 1988 Mar 22;444(2):320-4. doi: 10.1016/0006-8993(88)90940-7.
The 3-day sinoaortic-denervated (SAD) rat has a reduced concentration of dopamine (DA) in caudate-putamen (CP) (Brain Research, 299 (1984) 380-383). This suggested that the nigrostriatal system processes input from the cardiovascular system via arterial baroreceptor pathways. In this study we compared regional DA levels in CP of SAD and sham-operated (SO) rats 3 and 7 days after surgery. Bilateral CP samples were obtained by micropunch from consecutive frozen brain sections. Samples were taken throughout the entire length of CP from dorsal, lateral and ventral areas. Punches of two consecutive sections were pooled and assayed for DA and norepinephrine (NE). SO rats showed a rostro-caudal gradient of DA in all 3 areas. Three-day SAD rats had significantly lower cumulative DA concentrations in each of the 3 CP areas. The concentrations in each region of the 3 areas were also reduced and about half of them achieved statistical significance. SO rats did not show a gradient of NE. Cumulative NE was significantly lower in dorsal and ventral areas of SAD rats. In 7 days SAD rats, DA values were no longer reduced, but were significantly elevated in dorsal but unchanged in ventral areas. These data support evidence of nigrostriatal interaction with baroreceptor pathways.
3日龄去窦主动脉神经（SAD）大鼠尾状核-壳核（CP）中的多巴胺（DA）浓度降低（《脑研究》，299（1984）380 - 383）。这表明黑质纹状体系统通过动脉压力感受器通路处理来自心血管系统的输入。在本研究中，我们比较了手术3天和7天后SAD大鼠与假手术（SO）大鼠CP中的区域DA水平。通过对连续的冰冻脑切片进行微量打孔获得双侧CP样本。样本取自CP整个长度的背侧、外侧和腹侧区域。将连续两个切片的打孔样本合并，并测定DA和去甲肾上腺素（NE）。SO大鼠在所有3个区域均显示出DA的头-尾梯度。3日龄SAD大鼠在3个CP区域中的每一个区域的累积DA浓度均显著降低。3个区域中每个区域的浓度也降低，其中约一半达到统计学意义。SO大鼠未显示出NE的梯度。SAD大鼠背侧和腹侧区域的累积NE显著降低。在7日龄SAD大鼠中，DA值不再降低，但在背侧显著升高，而在腹侧区域不变。这些数据支持了黑质纹状体与压力感受器通路相互作用的证据。
