Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.
Insititute for Food and Drug Control, No. 1086 Danxi East Road, Jinhua, 321000, China.
Carbohydr Polym. 2021 Apr 15;258:117662. doi: 10.1016/j.carbpol.2021.117662. Epub 2021 Jan 21.
The functionality and property of pectin are correlated with its structure which is affected by the extraction method used. In this study, three different methods of extracting pectin, the microwave-assisted extraction (MAE), the ultrasonic-assisted extraction (UAE) and the conventional heating extraction (CHE), were used at three different temperatures with both an acid and alkali extraction solution. It was found that temperature mainly influenced pectin yield, while pectin structures and physicochemical properties were affected by the pH condition and extraction technology. The alkali-extraction with MAE and UAE at short time promoted the yield of low-ester pectin. Monosaccharide composition analysis showed a high galacturonic acid (GalA) content in the pectin derived from MAE and UAE. The high viscosity and desirable viscoelastic properties of the acid-MAE pectin were due to its large molecular weight and particle size. The results contribute to our understanding of the association among pectin extraction, structure and properties.
果胶的功能和性质与其结构有关,而结构又受到提取方法的影响。在这项研究中,使用了三种不同的提取果胶的方法:微波辅助提取(MAE)、超声辅助提取(UAE)和传统加热提取(CHE),并分别在三种不同的温度下使用酸提取液和碱提取液。结果发现,温度主要影响果胶的产率,而果胶的结构和理化性质则受到 pH 值条件和提取技术的影响。短时间内的 MAE 和 UAE 碱提取有助于提高低酯果胶的产率。单糖组成分析表明,MAE 和 UAE 提取的果胶中半乳糖醛酸(GalA)含量较高。酸-MAE 果胶具有较高的粘度和理想的黏弹性,这归因于其较大的分子量和粒径。研究结果有助于我们理解果胶提取、结构和性质之间的关系。