Chemistry and Biochemistry Program, University of Northern British Columbia, Prince George, British Columbia, V2N 4Z9, Canada.
Ecosystem Science and Management Program, University of Northern British Columbia, Prince George, British Columbia, V2N 4Z9, Canada.
Carbohydr Polym. 2021 Apr 15;258:117700. doi: 10.1016/j.carbpol.2021.117700. Epub 2021 Jan 27.
An immuno-stimulatory polysaccharide (EtISPFa) was purified from water extract of the fungus Echinodontium tinctorium. EtISPFa has an estimated weight average molecular weight (M) of 1354 kDa and is composed of glucose (66.2 %), glucuronic acid (10.1 %), mannose (6.7 %), galactose (6.4 %), xylose (5.6 %), rhamnose (3.1 %), fucose (1.8 %), and arabinose (0.2 %). It has multiple glycosidic linkages, with 3-Glcp (19.8 %), 4-GlcpA (10.8 %), 6-Glcp (10.7 %), and 3,6-Glcp (8.7 %) being the most prominent. NMR analysis showed that EtISPFa has a backbone containing mostly of 3-substituted β-glucopyranose with 4-substituted glucopyranosyluronic acid. Short side chains consisting of an average of two β-glycopyranose residues, connected through 1→6 linkages, are attached to the 6-position of about every 4th or 5th backbone glucose residue. EtISPFa is a novel glucuronic acid-containing β-glucan capable of significantly inducing the production of cytokines IL-17, IL-16, MIP-2, G-CSF,GM-CSF, LIF, MIP-1α, MIP-1β, and RANTES in vitro. EtISPFa should be further explored for its immuno-stimulatory activity in vivo.
一种免疫刺激多糖（EtISPFa）从真菌彩绒革盖菌的水提取物中纯化得到。EtISPFa 的重均分子量（M）估计为 1354kDa，由葡萄糖（66.2%）、葡萄糖醛酸（10.1%）、甘露糖（6.7%）、半乳糖（6.4%）、木糖（5.6%）、鼠李糖（3.1%）、岩藻糖（1.8%）和阿拉伯糖（0.2%）组成。它具有多种糖苷键，其中 3-Glcp（19.8%）、4-GlcpA（10.8%）、6-Glcp（10.7%）和 3,6-Glcp（8.7%）最为突出。NMR 分析表明，EtISPFa 具有主要含有 3-取代β-吡喃葡萄糖的主链，以及 4-取代的吡喃葡萄糖醛酸。短侧链由平均两个通过 1→6 键连接的β-吡喃糖苷残基组成，连接到主链中每个第 4 或第 5 个葡萄糖残基的 6-位。EtISPFa 是一种新型的含葡萄糖醛酸的β-葡聚糖，能够显著诱导细胞因子 IL-17、IL-16、MIP-2、G-CSF、GM-CSF、LIF、MIP-1α、MIP-1β和 RANTES 的体外产生。应该进一步探索 EtISPFa 在体内的免疫刺激活性。
