用于快速检测革兰氏阴性杆菌中碳青霉烯酶活性的比色法检测评估：MAST® PAcE检测
Evaluation of a colorimetric test for the rapid detection of carbapenemase activity in Gram negative bacilli: the MAST® PAcE test.
作者信息
Rezzoug Inès, Emeraud Cécile, Sauvadet Aimie, Cotellon Garance, Naas Thierry, Dortet Laurent
机构信息
Department of Bacteriology-Hygiene, Bicêtre Hospital, Assistance Publique - Hôpitaux de Paris, Le Kremlin-Bicêtre, France.
UMR-S 1184, Paris-Saclay University, Le Kremlin-Bicêtre, France.
出版信息
Antimicrob Agents Chemother. 2023 May 1;95(5). doi: 10.1128/AAC.02351-20. Epub 2021 Feb 16.
Abstract
The MAST Carba PAcE test is a colorimetric test used to detect carbapenemase-producing Gram-negative bacilli from cultured colonies. The performances of this test were compared to β-CARBA™, Carba NP test and RAPIDEC CARBA NP tests using a collection of 280 characterized isolates. Sensitivity and specificity of the MAST Carba PAcE test were 79.8% (95%IC: 73.3%-85.1%) and 98.9% (95%IC: 92.9%-99.9%). The MAST Carba PAcE sensitivity was the lowest mainly due to interpretation difficulties (particularly OXA-48-like).
摘要
MAST Carba PAcE试验是一种比色试验，用于从培养菌落中检测产碳青霉烯酶的革兰氏阴性杆菌。使用一组280株已鉴定的分离株，将该试验的性能与β-CARBA™、Carba NP试验和RAPIDEC CARBA NP试验进行了比较。MAST Carba PAcE试验的敏感性和特异性分别为79.8%（95%置信区间：73.3%-85.1%）和98.9%（95%置信区间：92.9%-99.9%）。MAST Carba PAcE的敏感性最低，主要是由于解读困难（尤其是OXA-48样）。
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