Li Ling, Zhang Mengge, Wang Wenjia, Xia Ruirui, Ma Yanan, Wei Xin, Wang Xinhua, Sun Xiaomin, Xie Xiaohong, Xie Shiling, Wang Mingyu, Xu Hai
State Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao, Shandong, China.
Shandong Provincial Key Laboratory of Water Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Shandong University, Qingdao, Shandong, China.
Antimicrob Agents Chemother. 2023 May 1;95(5). doi: 10.1128/AAC.02010-20. Epub 2021 Feb 16.
Two novel IS-associated genes, and , were identified from trimethoprim (TMP)-resistant strains and were shown to confer high level TMP resistance (MIC ≥ 1024 mg/L) when cloned into These genes were hosted by complex class 1 integrons suggesting their potentials for dissemination. Analysis of enzymatic parameters and TMP affinity were performed, suggesting that the mechanism of TMP resistance for these novel DHFRs is the reduction of binding with TMP.
从耐甲氧苄啶（TMP）菌株中鉴定出两个与整合子相关的新基因 和 ，当将它们克隆到 中时，显示出赋予高水平的TMP抗性（MIC≥1024mg/L）。这些基因由复杂的1类整合子携带，表明它们具有传播的潜力。对酶学参数和TMP亲和力进行了分析，表明这些新型二氢叶酸还原酶（DHFRs）的TMP抗性机制是与TMP结合的减少。
