Laboratory of Food Function and Biochemistry, Department of Food Science and Technology, National Research and Development Agency, Japan Fisheries Research and Education Agency, National Fisheries University, Shimonoseki, Japan.
Laboratory of Bioorganic Chemistry, Graduate School of Bioresources, Mie University, Tsu, Japan.
J Food Biochem. 2021 Apr;45(4):e13659. doi: 10.1111/jfbc.13659. Epub 2021 Feb 17.
We previously found a lipophilic fraction of the methanol/chloroform extract of a brown alga, Eisenia nipponica, that had an antiallergic effect in a murine ear swelling test. In this study, we purified the active component from the lipophilic fraction using high performance liquid chromatography and analyzed the mass and nuclear magnetic resonance spectra. This uncovered the phlorotannin dieckol, which exhibited antiallergic effects in an ear swelling test using mice sensitized by arachidonic acid, 12-O-tetradecanoylphorbol-13-acetate, and oxazolone. Mechanistic investigations indicated that dieckol suppressed degranulation, chemical mediator release, and the expression of mRNA such as cyclooxygenase-2, interleukin-6, and tumor necrosis factor-α in rat basophilic leukemia-2H3 cells. In summary, we isolated dieckol from E. nipponica and demonstrated its antiallergic mechanisms. PRACTICAL APPLICATIONS: As the incidence of allergies increases worldwide, so too does the demand for food components with antiallergic and anti-inflammatory properties. Given this trend, we focused on a brown alga that displays a variety of bioactivities. Here, we have isolated dieckol from the antiallergic lipophilic fraction of E. nipponica and found that it possesses diverse physiological activities that may prevent lifestyle-related diseases. Consequently, dieckol or the alga containing this phlorotannin could be used as a health food ingredient to combat not only allergies, but also variety of disorders including the undesirable effects of aging.
我们之前发现，一种褐藻——日本鳗草甲醇/氯仿提取物的亲脂部分在小鼠耳部肿胀试验中具有抗过敏作用。在这项研究中，我们使用高效液相色谱法从亲脂部分中纯化出活性成分，并分析了其质量和核磁共振谱。结果表明，这种成分是二苯甲酮型岩藻多酚——没食子酸二酯，它对由花生四烯酸、12-O-十四烷酰佛波醇-13-醋酸酯和恶唑酮诱导的小鼠耳部肿胀试验具有抗过敏作用。机制研究表明，没食子酸二酯可抑制肥大细胞脱颗粒、化学介质释放以及环氧化酶-2、白细胞介素-6 和肿瘤坏死因子-α mRNA 的表达。综上所述，我们从日本鳗草中分离出没食子酸二酯，并证实了其抗过敏机制。实际应用：随着全球过敏发病率的增加，对抗过敏和抗炎特性的食物成分的需求也在增加。鉴于这一趋势，我们关注了一种具有多种生物活性的褐藻。在这里，我们从日本鳗草的抗过敏亲脂部分中分离出没食子酸二酯，并发现它具有多种生理活性，可能预防与生活方式相关的疾病。因此，没食子酸二酯或含有这种岩藻多酚的藻类可以用作保健品成分，不仅可以对抗过敏，还可以对抗多种疾病，包括衰老带来的不良影响。
