School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, P.R. China.
College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou, P.R. China.
J Food Biochem. 2021 Apr;45(4):e13661. doi: 10.1111/jfbc.13661. Epub 2021 Feb 17.
The degraded polysaccharides from Porphyra yezoensis (DPPY) prepared using the H O -Vc method under optimized conditions were isolated and purified by DEAE Cellulose-52, and Sephadex G-100, providing four pure components, namely, DPPY-0, DPPY-0.1, DPPY-0.3, and DPPY-0.5. Their relative molecular weights were measured to be 10.8, 10.7, 18.7, and 35.5 kDa, respectively. GC-MS analysis revealed that all the four fractions were mainly composed of galactose, together with a small portion of glucose, mannose, xylose, and rhamnose. Structural analysis revealed that the purified polysaccharides mainly possess a backbone of (1 → 3)-β-D-galactose (1 → 4)-3,6-anhydro-α-L-galactopyranose (G-A) units and (1 → 3)-β-D-galactose (1 → 4)-α-L-galactose-6-sulfate (G-L6S) units. They were found to promote the proliferation of RAW264.7 macrophages and enhance phagocytosis of the RAW264.7 cells. Antioxidant assays indicated that DPPY-0.5 possessed the most potent reducing power and free radical scavenging ability among the four purified polysaccharides. High sulfate content and proper molecular weight of these fractions are favorable to their immunomodulatory and antioxidant activities. PRACTICAL APPLICATIONS: Porphyra yezoensis, common economic red algae widely distributed in East Asian countries, contains a high content of polysaccharides with a variety of biological activities. However, P. yezoensis polysaccharide (PPY) has not been well utilized due to the relatively low biological activities and lack of understanding of its structure-activity relationship. Thus, it is necessary to improve the bioactivities and elucidate the structure-activity relationship of this polysaccharide for its practical use. In the present work, four purified fractions (DPPY-0, DPPY-0.1, DPPY-0.3, and DPPY-0.5) were isolated from the degraded P. yezoensis polysaccharide, and were investigated for their antioxidant and immunoregulatory activities. The results of the present work will lay a foundation for the application of the degraded P. yezoensis polysaccharide in the food industry as a functional food ingredient.
使用 HO-Vc 法在优化条件下从紫菜（DPPY）中制备的降解多糖，通过 DEAE 纤维素-52 和 Sephadex G-100 进行分离和纯化，得到四种纯组分，分别为 DPPY-0、DPPY-0.1、DPPY-0.3 和 DPPY-0.5。它们的相对分子量分别测量为 10.8、10.7、18.7 和 35.5 kDa。GC-MS 分析表明，所有四个馏分主要由半乳糖组成，同时含有少量葡萄糖、甘露糖、木糖和鼠李糖。结构分析表明，纯化的多糖主要具有（1→3）-β-D-半乳糖（1→4）-3,6-脱水-α-L-半乳糖吡喃糖（G-A）单元和（1→3）-β-D-半乳糖（1→4）-α-L-半乳糖-6-硫酸盐（G-L6S）单元。它们被发现可促进 RAW264.7 巨噬细胞的增殖，并增强 RAW264.7 细胞的吞噬作用。抗氧化测定表明，在四种纯化多糖中，DPPY-0.5 具有最强的还原能力和自由基清除能力。这些馏分的高硫酸根含量和适当的分子量有利于其免疫调节和抗氧化活性。实际应用：紫菜是一种广泛分布于东亚国家的经济红藻，含有丰富的具有多种生物活性的多糖。然而，由于紫菜多糖（PPY）的生物活性相对较低，且其结构-活性关系尚不清楚，因此其尚未得到很好的利用。因此，有必要提高这种多糖的生物活性并阐明其结构-活性关系，以便实际应用。在本工作中，从降解紫菜多糖中分离得到四种纯化级分（DPPY-0、DPPY-0.1、DPPY-0.3 和 DPPY-0.5），并对其抗氧化和免疫调节活性进行了研究。本工作的结果将为在食品工业中作为功能性食品成分应用降解紫菜多糖奠定基础。
