Liu Yingying, Meng Yan, Ji Haozhen, Guo Jianhang, Shi Miaomiao, Lai Feiliao, Ji Xiaolong
College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450001, China.
National & Local Joint Engineering Research Center of Cereal-Based Foods (Henan), Zhengzhou 450001, China.
Food Chem X. 2024 Oct 20;24:101908. doi: 10.1016/j.fochx.2024.101908. eCollection 2024 Dec 30.
This study used an ultrasonically accelerated metal-free Fenton (HO-V system) reaction to promote water-extracted degrading polysaccharides from (DZMP). A novel jujube polysaccharide (DPZMP3) was obtained by degradation using DEAE-Sepharose Fast Flow and Sephacryl S-100 column chromatography. Methylation analysis, HPGPC, ion chromatography, FT-IR, and NMR spectroscopies were used to clarify the chemical structures of DPZMP3. Monosaccharide compositional analysis of DPZMP3 revealed the presence of Rha, Ara, Gal, and GalA at a molar ratio of 1.00:1.49:1.60:7.68, and the HPGPC data demonstrated the average of 34.3 kDa. Based on the structural and linkage research using NMR spectroscopy and GC-MS, it was determined that DPZMP3 was a homogalacturonan pectic polysaccharide with a (1 → 4)-Gal branch at C-6 and a small amount of Ara and Rha residues. The ultrasonic-aided Fenton treatment did not significantly alter the structure of DPZMP3. It may also be useful for DZMP and enhancing their antioxidant activity . The current study's findings could pave the way for the food sector to use jujube polysaccharides obtained by degradation as a functional food component.
本研究采用超声加速无金属芬顿（HO-V体系）反应来促进从[具体物质]中提取的水降解多糖（DZMP）。通过使用DEAE-琼脂糖快速流动柱和Sephacryl S-100柱色谱法降解，获得了一种新型枣多糖（DPZMP3）。采用甲基化分析、高效凝胶渗透色谱（HPGPC）、离子色谱、傅里叶变换红外光谱（FT-IR）和核磁共振光谱来阐明DPZMP3的化学结构。DPZMP3的单糖组成分析表明，鼠李糖（Rha）、阿拉伯糖（Ara）、半乳糖（Gal）和半乳糖醛酸（GalA）的摩尔比为1.00:1.49:1.60:7.68，HPGPC数据显示其平均分子量为34.3 kDa。基于使用核磁共振光谱和气相色谱-质谱联用（GC-MS）的结构和连接研究，确定DPZMP3是一种同型半乳糖醛酸果胶多糖，在C-6位有一个（1→4）-半乳糖分支以及少量的阿拉伯糖和鼠李糖残基。超声辅助芬顿处理并未显著改变DPZMP3的结构。它可能对DZMP也有用，并能增强其抗氧化活性。当前研究结果可为食品行业将降解获得的枣多糖用作功能性食品成分铺平道路。
