Chen Yujiao, He Senhao, Wang Yinghao, Hu Chuanming, Cheng Weitao, Zhou Lingjie, Ji Nanjing, Chen Haihong, Shen Xin
Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Key Laboratory of Marine Biotechnology, Jiangsu Ocean University, Lianyungang 222005, China.
Co-Innovation Center of Jiangsu Marine Bio-Industry Technology, Jiangsu Ocean University, Lianyungang 222005, China.
Int J Mol Sci. 2024 Nov 30;25(23):12894. doi: 10.3390/ijms252312894.
, a marine red algae species, has significant economic and ecological value. However, phosphorus (P) deficiency has emerged as a growing concern in many cultivation regions, negatively impacting its growth. To adapt to P deficiency, algae have evolved various strategies, including using dissolved organic phosphorus (DOP) sources to sustain growth. Despite its prevalence as a form of DOP, the utilization mechanism of glucose-6-phosphate (G6P) by remains unclear. In this study, the physiological and transcriptional responses of to P deficiency and G6P supplementation were examined. The results demonstrated that prolonged P deficiency significantly inhibited the growth of and had a negative impact on physiological indicators such as photosynthetic pigments and antioxidant enzyme activity. However, G6P treatment gradually alleviated these adverse effects over time. Both P deficiency and G6P treatment were associated with increased expression of genes involved in signal transduction and P starvation responses while concurrently downregulating genes related to photosynthesis and antioxidant defenses. In contrast, the suppression of gene expression was less significant under G6P treatment. This study elucidates the adaptive strategies of in response to P deficiency and clarifies the regulatory pathways involved in G6P utilization, providing novel insights into its P nutrient acquisition and metabolic regulation.
一种海洋红藻物种具有重要的经济和生态价值。然而，磷（P）缺乏在许多养殖区域已成为一个日益受到关注的问题，对其生长产生负面影响。为了适应磷缺乏，藻类已经进化出各种策略，包括利用溶解有机磷（DOP）来源来维持生长。尽管葡萄糖 - 6 - 磷酸（G6P）作为一种DOP形式普遍存在，但[藻类名称]对其利用机制仍不清楚。在本研究中，研究了[藻类名称]对磷缺乏和补充G6P的生理和转录反应。结果表明，长期磷缺乏显著抑制了[藻类名称]的生长，并对光合色素和抗氧化酶活性等生理指标产生负面影响。然而，随着时间的推移，G6P处理逐渐缓解了这些不利影响。磷缺乏和G6P处理均与参与信号转导和磷饥饿反应的基因表达增加有关，同时下调与光合作用和抗氧化防御相关的基因。相比之下，在G6P处理下基因表达的抑制作用较小。本研究阐明了[藻类名称]对磷缺乏的适应策略，并阐明了G6P利用所涉及的调控途径，为其磷营养获取和代谢调控提供了新的见解。
