Hu Dandan, Baskin Jerry M, Baskin Carol C, Liu Rong, Yang Xuejun, Huang Zhenying
State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China.
Key Laboratory for Humid Subtropical Eco-Geographical Processes of the Ministry of Education, School of Geographical Sciences, Fujian Normal University, Fuzhou 350007, China.
Mol Plant Microbe Interact. 2021 May;34(5):538-546. doi: 10.1094/MPMI-01-21-0014-FI. Epub 2021 Apr 12.
Seed mucilage plays important roles in the adaptation of desert plants to the stressful environment. is an important pioneer plant in the Central Asian cold desert, and it produces a large quantity of seed mucilage. Seed mucilage of can be degraded by soil microbes, but it is unknown which microorganisms can degrade mucilage or how the mucilage-degrading microorganisms affect rhizosphere microbial communities or root nutrients. Here, mucilage-degrading microorganisms were isolated from the rhizosphere of , were screened by incubation with mucilage stained with Congo red, and were identified by sequencing and phylogenetic analyses. Fungal-bacterial networks based on high-throughput sequencing of rhizosphere microbes were constructed to explore the seasonal dynamic of interactions between a mucilage-degrading microorganism and its closely related microorganisms. The structural equation model was used to analyze effects of the mucilage-degrading microorganism, rhizosphere fungal-bacterial communities, and soil physicochemical properties on root C and N. The fungus was identified as a mucilage-degrading microorganism. Relative abundance of the mucilage-degrading fungus (MDF) was highest in May. Subnetworks showed that the abundance of fungi and bacteria closely related to the MDF also were highest in May. Interactions between the MDF and related fungi and bacteria were positive, which might enhance mucilage degradation. In addition, the MDF might regulate root C and N by affecting rhizosphere microbial community structure. Our results suggest that MDF from the rhizosphere strengthens the plant-soil-microbe continuum, thereby potentially regulating microbial interactions and root nutrients of .[Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
种子黏液在沙漠植物适应胁迫环境中发挥着重要作用。[植物名称]是中亚寒冷沙漠中的一种重要先锋植物，它能产生大量种子黏液。[植物名称]的种子黏液可被土壤微生物降解，但尚不清楚哪些微生物能够降解黏液，以及黏液降解微生物如何影响根际微生物群落或根系养分。在此，从[植物名称]根际分离出黏液降解微生物，通过与刚果红染色的黏液一起培养进行筛选，并通过测序和系统发育分析进行鉴定。基于根际微生物高通量测序构建真菌-细菌网络，以探索黏液降解微生物与其密切相关微生物之间相互作用的季节动态。使用结构方程模型分析黏液降解微生物、根际真菌-细菌群落和土壤理化性质对根系碳和氮的影响。鉴定出真菌[具体真菌名称]为一种黏液降解微生物。黏液降解真菌（MDF）的相对丰度在5月最高。子网分析表明，与MDF密切相关的真菌和细菌的丰度在5月也最高。MDF与相关真菌和细菌之间的相互作用为正，这可能会增强黏液降解。此外，MDF可能通过影响根际微生物群落结构来调节根系碳和氮。我们的结果表明，来自根际的MDF加强了植物-土壤-微生物连续体，从而可能调节[植物名称]的微生物相互作用和根系养分。[公式：见正文] 版权所有© 2021作者。这是一篇根据知识共享署名-非商业性使用-禁止演绎4.0国际许可协议分发的开放获取文章。
