Tanaka A, Maki A, Kamiyama Y, Yamaoka Y, Ozawa K, Orii Y
Second Department of Surgery, Faculty of Medicine, Kyoto University, Japan.
Clin Chim Acta. 1988 Feb 29;172(1):93-9. doi: 10.1016/0009-8981(88)90124-6.
A spectrophotometric method was developed to determine cytochromes in human liver mitochondria contaminated with hemoglobin. The influence of hemoglobin on the measurement was canceled by keeping hemoglobin in the carbon monoxide bound form throughout determination. Mitochondria were solubilized by 2% sodium cholate, and cytochromes were reduced enzymatically with glutamate and succinate as substrates to the maximal extent. The amount of cytochromes determined spectrophotometrically was linearly correlated with mitochondrial protein at least up to the extent of 8 mg/ml.
开发了一种分光光度法来测定被血红蛋白污染的人肝线粒体中的细胞色素。在整个测定过程中，通过使血红蛋白保持一氧化碳结合形式来消除血红蛋白对测量的影响。线粒体用2%的胆酸钠溶解，细胞色素以谷氨酸和琥珀酸为底物通过酶促反应最大程度地还原。通过分光光度法测定的细胞色素量与线粒体蛋白至少在8mg/ml的范围内呈线性相关。
