Sato N, Kamada T, Abe H, Suematsu T, Kawano S, Hayashi N, Matsumura T, Hagihara B
Clin Chim Acta. 1977 Oct 15;80(2):243-51. doi: 10.1016/0009-8981(77)90031-6.
The mitochondrial and microsomal cytochromes in patients' livers obtained from needle biopsies have been analyzed using a sensitive spectrophotometric method. To determine simultaneously the concentration of these cytochromes from hemoglobin-contaminated liver, difference spectra of liver homogenates were taken at the temperature of liquid nitrogen before and after photolysis of carbon monoxide molecules from various hemoprotein-CO complexes. This method utilizes a characteristic property of hemoprotein-CO complexes, in which the reassociation of CO molecules with photolyzed hemoglobin, cytochrome P-450 and cytochrome alpha3 is temperature-dependent. The results in 69 cases with a variety of liver diseases have revealed that the concentrations of cytochromes alphaalpha3, P-450 and beta5 showed a huge variation in the injured livers as compared with those in the normal controls. The significance of the measurement of these cytochrome concentrations and their ratios is discussed.
采用一种灵敏的分光光度法，对通过肝穿刺活检获得的患者肝脏中的线粒体和微粒体细胞色素进行了分析。为了同时测定受血红蛋白污染的肝脏中这些细胞色素的浓度，在液氮温度下，对来自各种血红蛋白-一氧化碳复合物的一氧化碳分子进行光解前后，获取肝脏匀浆的差异光谱。该方法利用了血红蛋白-一氧化碳复合物的一个特性，即一氧化碳分子与光解后的血红蛋白、细胞色素P-450和细胞色素α3的重新结合是温度依赖性的。69例患有各种肝脏疾病的病例结果显示，与正常对照组相比，受损肝脏中细胞色素α3、P-450和β5的浓度有巨大差异。本文讨论了这些细胞色素浓度及其比率测定的意义。
