Department of Biology, Faculty of Science, Mahasarakham University, Kantharawichai District, Maha Sarakham, 44150, Thailand.
Walai Rukhavej Botanical Research Institute (WRBRI), Mahasarakham University, Kantharawichai District, Maha Sarakham, 44150, Thailand.
Trop Biomed. 2019 Dec 1;36(4):938-957.
Genetic variation based on mitochondrial cytochrome c oxidase I (COI) and II (COII) sequences was investigated for three black fly nominal species, Simulium metallicum Bellardi complex, S. callidum Dyar and Shannon, and S. ochraceum Walker complex, which are vectors of human onchocerciasis from Guatemala. High levels of genetic diversity were found in S. metallicum complex and S. ochraceum complex with maximum intraspecific genetic divergences of 11.39% and 4.25%, respectively. Levels of genetic diversity of these nominal species are consistent with species status for both of them as they are cytologically complexes of species. Phylogenetic analyses revealed that the S. metallicum complex from Guatemala divided into three distinct clades, two with members of this species from several Central and South American countries and another exclusively from Mexico. The Simulium ochraceum complex from Guatemala formed a clade with members of this species from Mexico and Costa Rica while those from Ecuador and Colombia formed another distinct clade. Very low diversity in S. callidum was found for both genes with maximum intraspecific genetic divergence of 0.68% for COI and 0.88% for COII. Low genetic diversity in S. callidum might be a consequence of the result being informative of only recent population history of the species.
基于线粒体细胞色素 c 氧化酶 I（COI）和 II（COII）序列的遗传变异，对三种黑蝇学名种进行了研究，即Simulium metallicum Bellardi 复合体、S. callidum Dyar and Shannon 和 S. ochraceum Walker 复合体，它们是危地马拉人类盘尾丝虫病的传播媒介。在 S. metallicum 复合体和 S. ochraceum 复合体中发现了高水平的遗传多样性，其最大种内遗传分歧分别为 11.39%和 4.25%。这些学名种的遗传多样性水平与其作为细胞复合体的物种地位一致。系统发育分析表明，来自危地马拉的 S. metallicum 复合体分为三个不同的分支，其中两个分支包含来自几个中美洲和南美洲国家的该物种成员，另一个分支则仅来自墨西哥。来自危地马拉的 S. ochraceum 复合体与来自墨西哥和哥斯达黎加的该物种成员形成一个分支，而来自厄瓜多尔和哥伦比亚的成员则形成另一个不同的分支。在 COI 和 COII 两个基因中，S. callidum 的遗传多样性都非常低，最大种内遗传分歧分别为 0.68%和 0.88%。S. callidum 的遗传多样性较低可能是由于该物种的种群历史信息仅来源于近期。
