School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia.
Vector Control Research Unit, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia.
Trop Biomed. 2019 Sep 1;36(3):687-693.
An investigation was undertaken for screening and isolating nematophagous-fungi from the faecal samples of various grazing animals and soils in Malaysia. Total of 111 faeces and 50 soil samples were collected and the samples were cultured on 2% water agar plates. The growth of nematophagous-fungi was stimulated by sprinkling-baiting technique. The conidia of suspected nematophagous-fungi were inoculated on 2% water agar plates. All isolated were maintained on 2% cornmeal agar plates. Verticillium spp., Fusarium spp. and Arthrobotrys spp. were identified from the faecal and soil samples. 62.5% of the faecal samples and 100% of the soil samples were shown to be positive with nematophagous-fungi. This study highlights the present of nematophagous-fungi population in faecal and soil samples. Much study remains to be done to better understanding some fungi especially their mode of action and their predatory behaviour against parasitic nematodes.
从马来西亚各种放牧动物的粪便样本和土壤中进行了调查，以筛选和分离食线虫真菌。共采集了 111 份粪便和 50 份土壤样本，并将样本培养在 2%水琼脂平板上。通过撒饵技术刺激食线虫真菌的生长。将疑似食线虫真菌的分生孢子接种在 2%水琼脂平板上。所有分离物均在 2%玉米粉琼脂平板上保存。从粪便和土壤样本中鉴定出了层出镰刀菌、镰刀菌和节丛孢属。62.5%的粪便样本和 100%的土壤样本显示出食线虫真菌呈阳性。本研究强调了食线虫真菌在粪便和土壤样本中的存在。为了更好地了解某些真菌，特别是它们对抗寄生线虫的作用方式和捕食行为，仍需要进行更多的研究。
