Rapid diagnosis of infection assisted by metagenomic next-generation sequencing in a HIV-negative patient.

is an opportunistic pathogenic fungus commonly reported in southeast Asia. infection predominantly occurs in patients with immunodeficiency and can be fatal if diagnosis and treatment were delayed. Conventional diagnosis of infection relies heavily on tissue culture and histologic analysis, which is time consuming and has limited positive rate. Rapid and accurate diagnosis of remains urgent for effective therapy and prevention. This case is the first reported infection in non-HIV patients in north China diagnosed by mNGS. The successful diagnosis of infection assistant by mNGS underlies the potential of this technique in rapid etiological diagnosis.