University of Pennsylvania, Department of Biology, Philadelphia, PA 19104, USA.
Rutgers University, Department of Biology, Camden, NJ 08102, USA.
STAR Protoc. 2021 Feb 5;2(1):100322. doi: 10.1016/j.xpro.2021.100322. eCollection 2021 Mar 19.
Mouse models are essential for studying pain neurobiology and testing pain therapeutics. The reliance on assays that only measure the presence, absence, or frequency of a reflex have limited the reliability of preclinical pain studies. Our high-speed videography protocol overcomes this by projecting the discrete sub-second kinematic behavioral features induced by hind paw stimulation onto a "mouse pain scale." This provides a more objective and robust pain measurement in mice by quantifying the quality of the stimulus-induced hind paw reflex. For complete details on the use and execution of this protocol, please refer to Abdus-Saboor et al. (2019).
鼠类模型对于疼痛神经生物学研究和疼痛治疗测试至关重要。依赖于仅测量反射存在、缺失或频率的检测方法，限制了临床前疼痛研究的可靠性。我们的高速录像方案通过将由后爪刺激引起的离散亚秒级运动行为特征投射到“鼠痛量表”上，克服了这一问题。这通过量化刺激诱导的后爪反射的质量，为小鼠提供了更客观、更稳健的疼痛测量。如需了解本方案的使用和执行的完整详细信息，请参考 Abdus-Saboor 等人（2019 年）的研究。
