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通过立体定位注射诱导小鼠大脑局部区域神经退行性变的方案。

Protocol to induce neurodegeneration in a local area of the mouse brain by stereotaxic injection.

机构信息

Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, China.

Department of Biomedical Sciences, City University of Hong Kong, Hong Kong, China.

出版信息

STAR Protoc. 2024 Sep 20;5(3):103243. doi: 10.1016/j.xpro.2024.103243. Epub 2024 Aug 2.

DOI:10.1016/j.xpro.2024.103243
PMID:39096497
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11342772/
Abstract

In vivo models of brain pathology are crucial for studying neurological diseases. Here, we present a protocol to induce a pathological condition in a mouse brain area by local injection of neurotoxic stimulus. We describe steps for preparing reagents, stereotaxic injection procedures to induce neurodegeneration in the hippocampus, and preparation of brain sections to examine the induced model. This protocol is useful for studying how local pathology affects other brain areas and neighbor cells and its functional consequences in behavior. For complete details on the use and execution of this protocol, please refer to Zhang et al..

摘要

体内脑病理学模型对于研究神经疾病至关重要。在这里，我们提出了一种通过局部注射神经毒性刺激物在小鼠脑区诱导病理状态的方案。我们描述了制备试剂、进行立体定位注射以在海马区诱导神经退行性变以及制备脑切片以检查诱导模型的步骤。该方案可用于研究局部病理学如何影响其他脑区和邻近细胞及其在行为中的功能后果。有关该方案的使用和执行的完整详细信息，请参阅 Zhang 等人的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8469/11342772/79d77d5b1ba3/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8469/11342772/0f7cf9af7e37/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8469/11342772/28cfb903c87b/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8469/11342772/79d77d5b1ba3/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8469/11342772/0f7cf9af7e37/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8469/11342772/28cfb903c87b/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8469/11342772/79d77d5b1ba3/gr3.jpg

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