Research Center for Medicinal Plant Resources, National Institutes of Biomedical Innovation, Health and Nutrition, 1-2 Hachimandai, Tsukuba, Ibaraki, 305-0843, Japan.
J Nat Med. 2021 Jun;75(3):707-716. doi: 10.1007/s11418-021-01494-1. Epub 2021 Feb 18.
A discriminant LC/MS quantitative analysis of ephedrine (EP) and pseudoephedrine (PEP) in Ephedrae herba was performed. Aerial parts of three Ephedra species were separated into internodes and extracted using Finger Masher with minimum loss. The contents of EP and PEP were measured by LC/MS/MS using the multiple reaction monitoring (MRM) method. Their contents in old-year branches were lower than those current-year branches and tended to be higher in the middle part than in the tip of each branch. The content ratio of EP and PEP was reversed in some branches depending on their extent of growth. In E. sinica, the contents were low at the first internode closest to the central main stem at each branch. The contents drastically increased from the second internode and were highest at the third internode. There was a strong correlation between the internode distance and alkaloid (EP + PEP) contents. The distribution of alkaloids in one internode was examined and the results showed that the part closest to the node had the lowest contents.
建立了一种 LC/MS 鉴别方法，用于定量分析麻黄中的麻黄碱（EP）和伪麻黄碱（PEP）。采用Finger Masher 提取法，以最小的损失将三种麻黄属植物的地上部分分为节间，并用 LC/MS/MS 法采用多反应监测（MRM）法进行测定。EP 和 PEP 的含量在老枝中低于当年生枝，且在每个枝的中部高于梢部。根据生长程度的不同，有些枝条中 EP 和 PEP 的含量比值发生了逆转。在中国麻黄中，每个枝最靠近中央主茎的第一节间含量较低。从第二节间开始含量急剧增加，第三节间含量最高。节间距离与生物碱（EP + PEP）含量之间存在很强的相关性。对一个节间的生物碱分布进行了考察，结果表明，靠近节的部分含量最低。
