一种改良的 H-NMR 定量法测定麻黄药材中麻黄碱类生物碱的含量。
A Modified H-NMR Quantification Method of Ephedrine Alkaloids in Ephedrae Herba Samples.
机构信息
School of Pharmacy, College of Medicine, National Cheng Kung University, Tainan 701, Taiwan.
School of Post-Baccalaureate Chinese Medicine, China Medical University, Taichung 404, Taiwan.
出版信息
Int J Mol Sci. 2023 Jul 10;24(14):11272. doi: 10.3390/ijms241411272.
A previous H-NMR method allowed the quantification of ephedrine alkaloids; however, there were some disadvantages. The cyclized derivatives resulted from the impurities of diethyl ether were identified and benzene was selected as the better extraction solvent. The locations of ephedrine alkaloids were confirmed with 2D NMR. Therefore, a specific H-NMR method has been modified for the quantification of ephedrine alkaloids. Accordingly, twenty Ephedrae Herba samples could be classified into three classes: (I) -like species; (II) -like species; (III) others (lower alkaloid contents). The results indicated that ephedrine and pseudoephedrine are the major alkaloids in plants, but the concentrations vary greatly determined by the plant species and the collection locations.
先前的 H-NMR 方法可以定量检测麻黄碱类生物碱，但存在一些缺点。我们鉴定出二乙醚杂质产生的环状衍生物，并选择苯作为更好的提取溶剂。我们通过 2D NMR 确定了麻黄碱类生物碱的位置。因此，对 H-NMR 方法进行了改进，用于定量检测麻黄碱类生物碱。由此，二十种麻黄属植物可分为三类：（I）-类似种；（II）-类似种；（III）其他（生物碱含量较低）。结果表明，麻黄碱和伪麻黄碱是植物中的主要生物碱，但由于植物种类和采集地点的不同，其浓度差异很大。
Zotero 插件