School of Pharmaceutical Sciences, Peking University, Beijing, China.
J Nat Med. 2011 Jul;65(3-4):623-8. doi: 10.1007/s11418-011-0528-8. Epub 2011 Apr 6.
In the present paper, a fast and economical HPLC method [on a Phenomenex Polar-RP column with a solution of (phosphoric acid:triethylamine:dibutylamine:water = 0.40:0.1:0.2:499.3) and methanol] is developed, and applied for the determination of norephedrine, norpseudoephedrine, ephedrine (E), pseudoephedrine (PE) and methylephedrine (ME) in 64 samples of three species from main habitats in China. Quantitation data showed that total alkaloid content in Ephedra equisetina Bge. (2.708 ± 0.642%) is higher than that in E. sinica Stapf. (1.365 ± 0.624%) and E. intermedia Schrenk et C. A. Mey. (1.537 ± 0.746%), but the range of total alkaloid content in each species is so wide that the ranges of the three species greatly overlap. The contents of E, PE and ME are different among the three species. The ratio E/total alkaloid content and ratio E/PE as well as E and ME contents can be used for identification of E. sinica, E. intermedia and E. equisetina from one another.
本文建立了一种快速、经济的高效液相色谱法（在 Phenomenex Polar-RP 柱上，采用（磷酸：三乙胺：二丁胺：水=0.40:0.1:0.2:499.3）和甲醇溶液），并应用于测定中国 64 个主要产地的三种麻黄属植物中去甲麻黄碱、去甲伪麻黄碱、麻黄碱（E）、伪麻黄碱（PE）和甲基麻黄碱（ME）的含量。定量数据表明，麻黄（2.708±0.642%）总生物碱含量高于中麻黄（1.365±0.624%）和木贼麻黄（1.537±0.746%），但各物种总生物碱含量的范围很宽，三个物种的范围有很大的重叠。三种麻黄属植物中 E、PE 和 ME 的含量不同。E/总生物碱含量比、E/PE 比以及 E 和 ME 的含量可用于鉴别中麻黄、木贼麻黄和麻黄。
