Accurate Monitoring of Mitochondrial HO by Robust SERS Nanoprobes with a Au-Se Interface.

Mitochondrial redox homeostasis plays a vital role in many biological processes. Hydrogen peroxide (HO), one of the most important components for the balance between oxidizing species and reducing species, also acts as the messenger of mitochondrial damage. Thus, an accurate quantitative detection of HO in mitochondria is very important for the evaluation of mitochondrial redox homeostasis. Here, we develop robust surface-enhanced Raman spectroscopy (SERS) nanoprobes based on Au nanoparticles as SERS substrate and functionalized carrier, which is further modified with a phenylboronic acid pinacol ester for specific HO response and a location peptides for mitochondrial targeting by creating a robust Au-Se interface. The SERS nanoprobes show good resistance to abundant thiol under biological conditions and superior performance for mitochondria HO monitoring in living cells than the SERS nanoprobes with the traditional Au-S interface, which enables us to achieve quantification of mitochondrial HO and obtain its real-time dynamic change under oxidative stress.