Division of Microbiology, Faculdade São Leopoldo Mandic, Campinas, São Paulo, Brazil.
Division of Oral Medicine, Faculdade São Leopoldo Mandic, Campinas, São Paulo, Brazil.
PLoS One. 2021 Feb 18;16(2):e0247029. doi: 10.1371/journal.pone.0247029. eCollection 2021.
Strategies to return to dental practice in pandemic times is a new challenge due to the generation and spread of potentially contaminated dispersion particles (PCDP) that may contain the SARS-CoV-2, the etiological factor of the COVID-19 disease. Due to the significant dispersion of PCDP in the dental environment, the use of equipment such as ultrasonic tips have been inadvisable during the pandemic. Several clinical procedures, however, benefit from the use of such equipment. Thus, using a microbial dispersion model of PCDP, the aim of this study was to compare the dispersion caused by the dental drill (DD) an ultrasonic tip (UT) alone and the UT coupled with a Spray control (SC) device. The DD, UT (with or without the SC) were activated for one minute having had the water from the reservoir replaced with a suspension of Lactobacillus casei Shirota (1.5 x 108 CFU/mL). Petri dishes containing MRS agar were positioned at 50cm, 100cm and 150cm from the headrest of the dental chair at different angles (0 degree and 90 degrees). At 50 cm, the mean CFU (standard deviation) of L. casei Shirota was 13554.60 (4071.03) for the DD, 286.67 (73.99) for the US (97.89% reduction), and 4.5 (0.58) CFU for the UT-SC (p < 0.0001), establishing a further 98.43% reduction between UT and UT with SC. The UT with SC model proved effective in reducing dispersion from the UT, endorsing its use as an additional strategy to reduce PCDP in the dental environment in times of pandemic.
在大流行时期,恢复牙科实践的策略是一项新的挑战,这是由于潜在污染分散颗粒(PCDP)的产生和传播所致,这些颗粒可能含有 SARS-CoV-2,即 COVID-19 疾病的病因。由于 PCDP 在牙科环境中的显著分散,因此在大流行期间不建议使用超声探头等设备。然而,一些临床程序受益于此类设备的使用。因此,使用 PCDP 的微生物分散模型,本研究的目的是比较牙科钻头(DD)和超声探头(UT)单独以及 UT 与喷雾控制(SC)装置结合使用引起的分散。DD、UT(带或不带 SC)在储水器中的水被含有干酪乳杆菌 Shirota(1.5 x 108 CFU/mL)的悬浮液取代后,激活一分钟。含有 MRS 琼脂的培养皿放置在头靠椅头枕 50cm、100cm 和 150cm 处,角度不同(0 度和 90 度)。在 50cm 处,L. casei Shirota 的平均 CFU(标准偏差)为 DD 为 13554.60(4071.03),US 为 286.67(73.99)(减少 97.89%),UT-SC 为 4.5(0.58)CFU(p<0.0001),UT 与 UT-SC 之间进一步减少 98.43%。UT-SC 模型证明可有效减少 UT 的分散,支持将其用作在大流行期间减少牙科环境中 PCDP 的附加策略。