底物决定了分离出的人皮脂腺合成中性脂质的速率和模式。

The substrate determines the rate and pattern of neutral lipid synthesized by isolated human sebaceous glands.

作者信息

Middleton B, Birdi I, Heffron M, Marsden J R

机构信息

Department of Biochemistry, University Medical School, Nottingham, England.

出版信息

FEBS Lett. 1988 Apr 11;231(1):59-61. doi: 10.1016/0014-5793(88)80702-6.

DOI:10.1016/0014-5793(88)80702-6

PMID:3360131

Abstract

Lipogenesis from different substrates was determined in isolated human sebaceous glands after 17-20 h in culture. Rates of total lipogenesis were 1003 +/- 141, 842 +/- 90, 481 +/- 57 pmol.h-1 gland-1 +/- SE from acetate, lactate and glucose, respectively, when present as sole substrates: the rate from glucose was significantly lower (P less than 0.01). Squalene synthesis was greatest from acetate at 479 +/- 44 pmol.h-1.gland-1; significantly higher than from lactate (281 +/- 45 pmol.h-1.gland-1) or glucose at 119 +/- 18 pmol.h-1.gland-1. Wax ester plus cholesterol ester synthesis showed similar dependence on substrate but triglyceride synthesis was unaffected. We conclude that the added substrate determines both the rate and pattern of non-polar lipid synthesized by isolated human sebaceous glands.

摘要

在培养17 - 20小时后，测定分离出的人皮脂腺中不同底物的脂肪生成情况。当乙酸盐、乳酸盐和葡萄糖分别作为唯一底物时，总脂肪生成速率分别为1003±141、842±90、481±57 pmol·h⁻¹·腺体⁻¹±标准误：葡萄糖的生成速率显著更低（P＜0.01）。角鲨烯合成以乙酸盐为底物时最高，为479±44 pmol·h⁻¹·腺体⁻¹；显著高于以乳酸盐为底物时（281±45 pmol·h⁻¹·腺体⁻¹）或以葡萄糖为底物时的119±18 pmol·h⁻¹·腺体⁻¹。蜡酯加胆固醇酯的合成对底物有相似的依赖性，但甘油三酯合成不受影响。我们得出结论，添加的底物决定了分离出的人皮脂腺合成非极性脂质的速率和模式。