人离体皮脂腺中的脂肪生成

Lipogenesis in isolated human sebaceous glands.

作者信息

Cassidy D M, Lee C M, Laker M F, Kealey T

出版信息

FEBS Lett. 1986 May 5;200(1):173-6. doi: 10.1016/0014-5793(86)80533-6.

Abstract

Lipogenesis in isolated human sebaceous glands from [U-14C]glucose, [U-14C]leucine, [U-14C]isoleucine, and [U-14C]valine has been determined by thin-layer chromatography. Total lipogenesis from 2 mmol/l [U-14C]glucose was 114.8 +/- 22.3 pmol/gland per h (mean +/- SE), with 53.8% being incorporated into triglycerides, 20.2% into squalene, 12.8% into phospholipids, 2.1% into cholesterol and 7.1% into wax monoester and cholesterol ester and 5% into di- and monoglycerides and free fatty acids. Total lipogenesis from 2 mmol/l [U-14C]leucine, 2 mmol/l [U-14C]isoleucine, and 2 mmol/l [U-14C]valine in the presence of 2 mmol/l glucose was 26, 29 and 9%, respectively, of that seen with 2 mmol/l glucose alone. The pattern of 14C distribution in the various lipid classes from the three U-14C-labelled branched-chain amino acids was not significantly different from that seen with [U-14C]glucose.

摘要

已通过薄层色谱法测定了从[U-14C]葡萄糖、[U-14C]亮氨酸、[U-14C]异亮氨酸和[U-14C]缬氨酸中分离出的人皮脂腺中的脂肪生成。来自2 mmol/l [U-14C]葡萄糖的总脂肪生成量为每小时114.8±22.3 pmol/腺体（平均值±标准误），其中53.8%掺入甘油三酯，20.2%掺入角鲨烯，12.8%掺入磷脂，2.1%掺入胆固醇，7.1%掺入蜡单酯和胆固醇酯，5%掺入甘油二酯和甘油单酯以及游离脂肪酸。在2 mmol/l葡萄糖存在的情况下，来自2 mmol/l [U-14C]亮氨酸、2 mmol/l [U-14C]异亮氨酸和2 mmol/l [U-14C]缬氨酸的总脂肪生成量分别为单独使用2 mmol/l葡萄糖时的26%、29%和9%。三种U-14C标记的支链氨基酸在各种脂质类别中的14C分布模式与[U-14C]葡萄糖的情况没有显著差异。