College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.
College of Food and Biological Engineering, Jimei University, Xiamen 361021, China.
Food Chem. 2021 May 30;345:128812. doi: 10.1016/j.foodchem.2020.128812. Epub 2020 Dec 7.
Due to complex matrixes and specific reagent deficiency, the rapid detection of histamine is still a challenge to date. Based on the high peroxidase-like activity of iron-cobalt co-doped carbon dots, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was established for histamine detection using the mimic enzyme labeled with histamine antibody (His-Ab). Through the competitive binding of the labeled His-Ab to solid-phase and sample antigens, histamine content was detected with a linear range of 2.5-150 μg mL. The detection limit based on 3σ/K was 0.50 mg kg, which was much lower than those of commercial His-kit and HPLC methods. The ic-ELISA method was applied to histamine detection in fish samples with the recovery of (103.4 ± 0.5)%, which was in accord with those of commercial His-kit and HPLC methods. The results indicated that the established ic-ELISA method was suitable for rapid detection of histamine in fish samples with high accuracy, sensitivity and stability.
由于复杂的基质和特定试剂的缺乏，至今为止，组胺的快速检测仍然是一个挑战。基于铁钴共掺杂碳点的高过氧化物酶样活性，本文建立了一种间接竞争酶联免疫吸附测定法（ic-ELISA），该方法通过用组胺抗体（His-Ab）标记模拟酶来检测组胺。通过标记的 His-Ab 与固相和样品抗原的竞争性结合，可以检测到 2.5-150μg mL 的线性范围。基于 3σ/K 的检测限为 0.50mg kg，远低于商业 His 试剂盒和 HPLC 方法。该 ic-ELISA 方法用于鱼类样品中的组胺检测，回收率为（103.4±0.5）%，与商业 His 试剂盒和 HPLC 方法一致。结果表明，所建立的 ic-ELISA 方法具有准确性高、灵敏度高和稳定性好的特点，适用于鱼类样品中组胺的快速检测。
