Lepper A W, Corner L A, Pearson C W
Aust Vet J. 1977 Jul;53(7):301-5. doi: 10.1111/j.1751-0813.1977.tb00236.x.
Five two-year-old heifers were each inoculated intravenously with 0.02 mg M. bovis strain AN5. Clinical, haematological and microbiological observations were made during the course of the experiment and antibody levels were measured before and after infection by means of the indirect immunofluorescent antibody (IFA) and bentonite flocculation tests. All cattle developed M. bovis infection varying in severity from peracute tuberculous pneumonia resulting in death within 33 days to chronic progressive generalised tuberculosis. Only cattle developing peracute or acute forms of tuberculosis showed marked haematological changes characterised by leucopenia with lymphopenia. Bacteraemia was detected in the two cattle with peracute tuberculosis 22 days after infection. Anti-mycobacterial antibody was detected after infection in all cattle but fluctuated markedly during the course of the disease. Of a total of 61 serum samples examined from all cattle after inoculation with M. bovis, only 38 were positive to the IFA test and 30 to the bentonite flocculation test. Only 18 were positive to both tests at any one time. IgM was the predominant type of anti-mycobacterial antibody detected by the IFA test and this was found to cross-react with M. avium in almost every sample.
给5头两岁的小母牛每头静脉注射0.02毫克牛分枝杆菌菌株AN5。在实验过程中进行临床、血液学和微生物学观察，并在感染前后通过间接免疫荧光抗体（IFA）和膨润土凝集试验测量抗体水平。所有牛都感染了牛分枝杆菌，严重程度各不相同，从在33天内导致死亡的超急性结核性肺炎到慢性进行性全身性结核病。只有发展为超急性或急性结核病形式的牛出现明显的血液学变化，其特征为白细胞减少伴淋巴细胞减少。在感染22天后，在两头患有超急性结核病的牛中检测到菌血症。感染后在所有牛中均检测到抗分枝杆菌抗体，但在疾病过程中波动明显。在接种牛分枝杆菌后从所有牛中检查的总共61份血清样本中，只有38份对IFA试验呈阳性，30份对膨润土凝集试验呈阳性。在任何时候只有18份对两种试验均呈阳性。IgM是IFA试验检测到的主要抗分枝杆菌抗体类型，并且发现几乎在每个样本中都与鸟分枝杆菌发生交叉反应。
