Mahdavipoor B, Asgharzadeh M, Hajibonabi F, Rashedi J, Pourostadi M, Bahador T N, Asadi N, Kafil H S, Barhaghi M H S
Department of Laboratory Science, Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Biotechnology Research Center, and Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Trop Biomed. 2018 Sep 1;35(3):619-626.
Planning to control tuberculosis requires identification of dominant strains in the region, transmission patterns and risk factors that are possible by using molecular genotyping techniques. The aim of this study is to determine the transmission of tuberculosis in the northwest of Iran in order to better understand the spread of disease in northwest of Iran. In this study, 194 positive mycobacterium cultivars in northwest of Iran were investigated using exact tandem repeat-variable number tandem repeats (ETR-VNTR) method. The ETR-VNTR method was identified 55 different patterns in 194 isolates, which contained 25 clusters and 30 unique patterns, and the largest cluster had 33 isolates, and discriminatory power of ETRVNTR method was determined 0.9322 in the examined samples. There are strains of Mycobacterium tuberculosis located in the northwest of Iran that infect people, and ETRVNTR method can be used as a first-line method to examine the dynamics of tuberculosis transmission.
规划结核病防控需要识别该地区的优势菌株、传播模式以及利用分子基因分型技术确定可能的风险因素。本研究的目的是确定伊朗西北部结核病的传播情况，以便更好地了解该病在伊朗西北部的传播。在本研究中，采用精确串联重复序列-可变数目串联重复序列（ETR-VNTR）方法对伊朗西北部的194株阳性分枝杆菌培养物进行了调查。ETR-VNTR方法在194株分离株中鉴定出55种不同模式，其中包括25个聚类和30个独特模式，最大的聚类有33株分离株，在所检测的样本中ETRVNTR方法的鉴别力为0.9322。伊朗西北部存在感染人群的结核分枝杆菌菌株，ETRVNTR方法可作为检测结核病传播动态的一线方法。
