Research Institute of Biotechnology & Medical Converged Science, Dongguk University-Seoul, Gyeonggi-do, 10326, Republic of Korea.
Department of Food Science and Biotechnology, Dongguk University-Seoul, Gyeonggi-do, 10326, Republic of Korea.
Int J Nanomedicine. 2021 Feb 11;16:977-987. doi: 10.2147/IJN.S290037. eCollection 2021.
The dragon tongue beans are a legume belonging to the Fabaceae family, are rich in protein, starch, fiber, and other micronutrients that have numerous health-promoting benefits. Its peel commonly the waste parts also contains lots of bioactive compounds.
In the current research, dragon tongue bean peels (DtbP) extract is tested for the existence of phytochemicals. Ag nanoparticles are biosynthesized using DtbP extract. The generated DtbP silver nanoparticle characterization was accomplished using UV-Vis spectral analysis, FTIR spectral analysis, SEM analysis, EDX analysis, XRD analysis, zeta potential, and DLS study. Furthermore, comparative assessment on multi-biological activities of the biosynthesized Ag nanoparticles was accomplished by employing cytotoxicity (inhibition against HepG cancer cells), antidiabetic (α-glucosidase inhibition assay), and antioxidant (free-radical scavenging) analysis.
The characterization result of the DtbP-AgNPs demonstrated that the AgNPs synthesized within 24 h. The AgNPs are nearly spherical. The biological effect assay of AgNPs displayed that DtbP-AgNPs is having significant cytotoxicity, antidiabetic, and moderate antioxidant effect. This study results as a whole report the biosynthesis of DtbP-AgNPs utilizing the legume dragon tongue bean waste peel and assessment of their multiple biological activities. The synthesized DtbP-AgNPs could serve as a potential candidate in the pharmaceutical industries in the formulation of drugs for the treatment of several medical ailments concerning cancer, diabetes, etc.
龙舌豆是豆科植物的一种，富含蛋白质、淀粉、纤维和其他对健康有益的微量营养素。其果皮通常是废物部分，也含有许多生物活性化合物。
在目前的研究中，测试了龙舌豆果皮（DtbP）提取物中是否存在植物化学物质。使用 DtbP 提取物生物合成银纳米粒子。通过紫外可见光谱分析、傅里叶变换红外光谱分析、SEM 分析、EDX 分析、XRD 分析、zeta 电位和 DLS 研究对生成的 DtbP 银纳米粒子进行了表征。此外，通过细胞毒性（抑制 HepG 癌细胞）、抗糖尿病（α-葡萄糖苷酶抑制试验）和抗氧化（自由基清除）分析，对生物合成的 Ag 纳米粒子的多种生物活性进行了比较评估。
DtbP-AgNPs 的表征结果表明，AgNPs 在 24 小时内合成。AgNPs 几乎呈球形。AgNPs 的生物效应试验表明，DtbP-AgNPs 具有显著的细胞毒性、抗糖尿病和适度的抗氧化作用。总的来说，这项研究报告了利用豆类龙舌豆废物果皮生物合成 DtbP-AgNPs 及其多种生物活性的评估。合成的 DtbP-AgNPs 可作为制药行业在治疗癌症、糖尿病等多种疾病的药物制剂中的潜在候选药物。
