UMR_S_1236, Univ Rennes, Inserm, Rennes, France.
Centre Hospitalier Universitaire de Rennes, Service Hématologie, Rennes, France.
Front Immunol. 2021 Feb 2;11:623993. doi: 10.3389/fimmu.2020.623993. eCollection 2020.
The monocyte/macrophage lineage has been shown to be involved in the promotion of a protumoral tumor microenvironment and resistance to treatment in B cell lymphomas. However, it is still poorly described at the single cell level, and tissue samples are not easily accessible. Thus, a detailed analysis of the circulating myeloid cell compartment in the different B lymphomas is needed to better understand the mechanisms of resistance to treatment and identify at risk patients. In this Perspective, we review current knowledge on the phenotypic and functional description of the circulating monocytic lineage in B cell lymphomas and provide first insights into the heterogeneity of these cell populations in health and lymphoma, using mass cytometry. Indeed, the monocytic compartment is a continuum more than distinct subpopulations, as demonstrated by our high-resolution approach, explaining the sometimes confusing and contradictory conclusions on the prognostic impact of the different populations, including monocytes and monocytic myeloid derived suppressor cells (M-MDSC). By identifying S100A9 monocytic cells as a potential biomarker in diffuse large B cell lymphoma (DLBCL) in this proof-of-concept preliminary study including a limited number of samples, we underline the potential of circulating myeloid regulatory cells as diagnostic and prognostic biomarkers in B-cell lymphomas.
单核细胞/巨噬细胞谱系已被证明参与了 B 细胞淋巴瘤中促进肿瘤前微环境和治疗抵抗的过程。然而，在单细胞水平上，其仍描述不足，且组织样本不易获取。因此，需要对不同 B 淋巴瘤中循环髓系细胞群进行详细分析，以更好地理解治疗抵抗的机制，并识别风险患者。在这篇观点文章中，我们综述了目前关于 B 细胞淋巴瘤中循环单核细胞谱系的表型和功能描述的知识，并使用质谱流式细胞术首次深入了解了这些细胞群体在健康和淋巴瘤中的异质性。事实上，正如我们的高分辨率方法所证明的那样，单核细胞群是一个连续体，而不是不同的亚群，这解释了不同群体（包括单核细胞和单核细胞来源的髓系抑制细胞[M-MDSC]）的预后影响有时令人困惑和矛盾的结论。通过在包括少数样本的这项初步概念验证研究中，将 S100A9 单核细胞鉴定为弥漫性大 B 细胞淋巴瘤（DLBCL）中的潜在生物标志物，我们强调了循环髓系调节细胞作为 B 细胞淋巴瘤诊断和预后生物标志物的潜力。
