Dachlan Ishandono, Kurniawan Hendy Satrya, Wicaksana Aditya, Fauzi Aditya Rifqi, Makrufardi Firdian, Seswandhana Rosadi
Plastic Reconstructive, and Aesthetic Surgery Division, Department of Surgery, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada/Dr. Sardjito Hospital, Yogyakarta, 55281, Indonesia.
Ann Med Surg (Lond). 2021 Feb 1;62:473-476. doi: 10.1016/j.amsu.2021.01.093. eCollection 2021 Feb.
Disturbance in the wound healing can cause the wound turn into a chronic wound, which histologically shows fibroblast senescence with weak proliferation ability. Mitomycin-C could block cell proliferation that causes cell senescence which is similar to the chronic wound morphology. Platelet-Rich Fibrin (PRF) contains a large number of platelets, leukocytes, cytokines and growth factors. This study aims to determine whether PRF could improve the fibroblast proliferation after treatment with Mitomycin-C.
Cultured normal human skin fibroblasts forth passage divided into five groups. The first group was treated with culture medium, and the second group with 10 μg/mL mitomycin-C for 2 h. The third, 4th and 5th group were treated with mitomycin-C for the same dose and period, then adding it with 100%, 50%, and 25% of PRF. The fibroblast proliferation was measured by MTT assay.
The fibroblast proliferation in the group with culture medium is 11.366,56 ± 4.073,32, meanwhile in the group with mitomycin-C treatment is 5.690,41 ± 2.834,22. The fibroblast proliferation in group with 100% PRF is 7.909,8 ± 3.392,19; group with 50% PRF 15.347,91 ± 8.413,02; and group with 25% PRF 13.449,56 ± 7.523,83. All of the PRF groups increased significantly compared to the group with Mitomycin-C treatment.
Platelet-Rich Fibrin can improve normal dermal fibroblast proliferation after treatment with mitomycin-C in vitro.
伤口愈合紊乱可导致伤口转变为慢性伤口，其组织学表现为成纤维细胞衰老，增殖能力减弱。丝裂霉素-C可阻断导致细胞衰老的细胞增殖，这与慢性伤口形态相似。富血小板纤维蛋白（PRF）含有大量血小板、白细胞、细胞因子和生长因子。本研究旨在确定PRF是否能在丝裂霉素-C处理后改善成纤维细胞增殖。
将培养的第四代正常人皮肤成纤维细胞分为五组。第一组用培养基处理，第二组用10μg/mL丝裂霉素-C处理2小时。第三、第四和第五组用相同剂量和时间的丝裂霉素-C处理，然后分别添加100%、50%和25%的PRF。通过MTT法检测成纤维细胞增殖。
培养基组的成纤维细胞增殖为11.36656±4.07332，而丝裂霉素-C处理组为5.69041±2.83422。100%PRF组的成纤维细胞增殖为7.9098±3.39219；50%PRF组为15.34791±8.41302；25%PRF组为13.44956±7.52383。与丝裂霉素-C处理组相比，所有PRF组均显著增加。
富血小板纤维蛋白可在体外改善丝裂霉素-C处理后的正常真皮成纤维细胞增殖。
