Garcia-Jacas Núria, Requena Jèssica, Massó Sergi, Vilatersana Roser, Blanché Cèsar, López-Pujol Jordi
Botanic Institute of Barcelona (IBB, CSIC-Ajuntament de Barcelona), Barcelona, Catalonia, Spain.
Departament de Biologia Animal, Biologia Vegetal i Ecologia, Facultat de Biociències, Universitat Autònoma de Barcelona, Bellaterra, Catalonia, Spain.
PeerJ. 2021 Feb 4;9:e10521. doi: 10.7717/peerj.10521. eCollection 2021.
(Apiaceae) is an extremely narrow endemic plant, which is considered as one of the species of most conservation concern in Catalonia (NW Mediterranean Basin). Given the accelerated fragmentation and reduction of population size (of over 90%), the environmental agency of Catalonia is currently preparing a recovery plan that includes reinforcements of the extant populations. The present study is aimed at providing the necessary knowledge to carry out genetically-informed translocations, by using microsatellites as genetic markers. Fourteen microsatellites have been specifically developed for , of which nine have been used. Besides the extant natural populations, the three ex situ collections that are known to exist of this species have also been studied, as they would be the donor sources for translocation activities. Our main finding is that levels of genetic diversity in the natural populations of are still high ( = 0.605), most likely as a result of a predominantly outcrossing mating system in combination with the limited time elapsed since the population decline. However, population fragmentation is showing the first genetic signs, as the values of genetic differentiation are relatively high, and two well-differentiated genetic lineages have been found even in such a narrow geographic range. These genetic results provide important information when designing conservation management measures.
（伞形科）是一种极度狭域分布的特有植物，被视为加泰罗尼亚地区（地中海盆地西北部）最受关注的保护物种之一。鉴于其种群加速碎片化且种群规模减少（超过90%），加泰罗尼亚环境机构目前正在制定一项恢复计划，其中包括对现存种群的强化措施。本研究旨在通过使用微卫星作为遗传标记，提供开展基于遗传信息的迁地保护所需的知识。已专门为[该物种名称未给出]开发了14个微卫星，其中9个已被使用。除了现存的自然种群外，还对已知存在的该物种的三个迁地保护收集群体进行了研究，因为它们将作为迁地保护活动的供体来源。我们的主要发现是，[该物种名称未给出]自然种群的遗传多样性水平仍然很高（平均等位基因数 = 0.605），这很可能是由于主要为异交的交配系统以及种群数量下降后经过的时间有限所致。然而，种群碎片化正显示出最初的遗传迹象，因为遗传分化值相对较高，而且即使在如此狭窄的地理范围内也发现了两个分化明显的遗传谱系。这些遗传结果在设计保护管理措施时提供了重要信息。
