Department of Aquaculture, Szent István University, Gödöllő, Páter Károly u. 1., Hungary.
Department of Biomedical Sciences, Center for Reproductive Genomics, Cornell University, Ithaca, NY, USA.
Methods Mol Biol. 2021;2218:37-47. doi: 10.1007/978-1-0716-0970-5_4.
Cryopreservation as a method that enables long-term storage of biological material has long been used for the conservation of valuable zebrafish genetic resources. However, currently, only spermatozoa of zebrafish can be successfully cryopreserved, while protocols for cryopreservation of eggs and embryos have not yet been fully developed. Transplantation of germline stem cells (GSCs) has risen as a favorable method that can bypass the current problem in cryopreservation of female genetic resources and can lead to reconstitution of fish species and lines through surrogate production. Here, we describe essential steps needed for the cryopreservation of spermatogonial stem cells (SSCs) and their utilization in the conservation of zebrafish genetic resources through SSC transplantation and surrogate production.
冷冻保存作为一种能够长期储存生物材料的方法，长期以来一直被用于保护有价值的斑马鱼遗传资源。然而，目前只有斑马鱼的精子可以成功地进行冷冻保存，而卵子和胚胎的冷冻保存方案尚未完全开发。生殖干细胞（GSCs）的移植作为一种有前途的方法，已经出现，可以绕过当前冷冻保存雌性遗传资源的问题，并通过代孕生产导致鱼类物种和品系的重建。在这里，我们描述了冷冻保存精原干细胞（SSCs）所需的基本步骤，并通过 SSC 移植和代孕生产利用 SSCs 来保护斑马鱼遗传资源。
