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糖蛋白聚糖-5 的亚细胞定位与人类间充质干细胞系 U3DT 的动态迁移有关。

Subcellular localization of glypican-5 is associated with dynamic motility of the human mesenchymal stem cell line U3DT.

机构信息

Section of Laboratory Equipment, National Institutes of Biomedical Innovation, Health and Nutrition, Ibaraki-city, Osaka, Japan.

Division of Bio-informational Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, Bunkyo, Tokyo, Japan.

出版信息

PLoS One. 2021 Feb 19;16(2):e0226538. doi: 10.1371/journal.pone.0226538. eCollection 2021.

DOI:10.1371/journal.pone.0226538
PMID:33606708
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7895401/
Abstract

Glypican-5 (GPC5) is a heparan sulfate proteoglycan (HSPG) localized to the plasma membrane. We previously reported that in the human mesenchymal stem cell line UE6E7T-3, GPC5 is overexpressed in association with transformation and promotes cell proliferation by acting as a co-receptor for Sonic hedgehog signaling. In this study, we found using immunofluorescence microscopy that in transformed cells (U3DT), GPC5 localized not only at primary cilia on the cell surface, but also at the leading edge of migrating cells, at the intercellular bridge and blebs during cytokinesis, and in extracellular vesicles. In each subcellular region, GPC5 colocalized with fibroblast growth factor receptor (FGFR) and the small GTPases Rab11 and ARF6, indicating that GPC5 is delivered to these regions by Rab11-associated recycling endosomes. These colocalizations suggest that GPC5 plays an important role in FGF2 stimulation of cell migration, which was abrogated by knockdown of GPC5. Our findings indicate that GPC5 plays a role in regulation of U3DT cell migration and provides several insights into the functions of GPC5 that could be elucidated by future studies.

摘要

Glypican-5 (GPC5) 是一种位于质膜上的硫酸乙酰肝素蛋白聚糖 (HSPG)。我们之前报道过，在人间充质干细胞系 UE6E7T-3 中，GPC5 与转化相关的过表达，并通过作为 Sonic hedgehog 信号转导的共受体促进细胞增殖。在这项研究中，我们通过免疫荧光显微镜发现，在转化细胞 (U3DT) 中，GPC5 不仅定位于细胞表面的初级纤毛上，而且还定位于迁移细胞的前缘、细胞分裂过程中的细胞间桥和小泡以及细胞外囊泡中。在每个亚细胞区域，GPC5 与成纤维细胞生长因子受体 (FGFR) 和小 GTPases Rab11 和 ARF6 共定位，表明 GPC5 通过 Rab11 相关的再循环内体被递送到这些区域。这些共定位表明 GPC5 在 FGF2 刺激细胞迁移中发挥重要作用，而 GPC5 的敲低则削弱了这种作用。我们的研究结果表明，GPC5 在调节 U3DT 细胞迁移中发挥作用，并为未来研究阐明 GPC5 的功能提供了一些见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de39/7895401/d98bdf4f4f23/pone.0226538.g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de39/7895401/2cea7045058d/pone.0226538.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de39/7895401/d29c96bea23b/pone.0226538.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de39/7895401/5474ffe0dd75/pone.0226538.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de39/7895401/c001e3615ba2/pone.0226538.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de39/7895401/4eebb4fbd90e/pone.0226538.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de39/7895401/c1d4fd676ebd/pone.0226538.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de39/7895401/90e99ce6cc47/pone.0226538.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de39/7895401/3246e93bacef/pone.0226538.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de39/7895401/14ace58743b4/pone.0226538.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de39/7895401/d57a0fa4e468/pone.0226538.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de39/7895401/d98bdf4f4f23/pone.0226538.g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de39/7895401/2cea7045058d/pone.0226538.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de39/7895401/d29c96bea23b/pone.0226538.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de39/7895401/5474ffe0dd75/pone.0226538.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de39/7895401/c001e3615ba2/pone.0226538.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de39/7895401/4eebb4fbd90e/pone.0226538.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de39/7895401/c1d4fd676ebd/pone.0226538.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de39/7895401/90e99ce6cc47/pone.0226538.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de39/7895401/3246e93bacef/pone.0226538.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de39/7895401/14ace58743b4/pone.0226538.g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de39/7895401/d57a0fa4e468/pone.0226538.g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/de39/7895401/d98bdf4f4f23/pone.0226538.g011.jpg

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本文引用的文献

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2
Visualizing and Quantifying the Effect of the Inhibition of HSP70 on Breast Cancer Cells Based on Laser Scanning Microscopy.基于激光扫描显微镜观察和量化热休克蛋白70（HSP70）抑制对乳腺癌细胞的影响
Technol Cancer Res Treat. 2018 Jan 1;17:1533033818785274. doi: 10.1177/1533033818785274.
3
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Dev Cell. 2017 Dec 4;43(5):577-587.e5. doi: 10.1016/j.devcel.2017.10.030. Epub 2017 Nov 22.
4
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Oncol Res. 2018 May 7;26(4):565-572. doi: 10.3727/096504017X15044461944385. Epub 2017 Sep 6.
5
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6
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7
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8
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