CYTOXLAB, Geneva University Hospitals, Geneva, Switzerland
School of Pharmaceutical Sciences, University of Geneva, Geneva, Switzerland.
Eur J Hosp Pharm. 2021 Mar;28(2):94-99. doi: 10.1136/ejhpharm-2019-001983. Epub 2019 Jul 17.
Optimise a wipe sampling procedure to evaluate the surface contamination for 23 antineoplastic drugs used in the hospital pharmacy.
The influence of various parameters (ie, sampling device, sampling solution, desorption modes) was evaluated using a validated liquid chromatography-mass spectrometry (LC-MS/MS) method able to quantify 23 antineoplastic drugs widely used in the hospital pharmacy: 5-fluorouracil, busulfan, cyclophosphamide, cytarabine, dacarbazine, daunorubicin, docetaxel, doxorubicin, epirubicin, etoposide, etoposide phosphate, fludarabine phosphate, ganciclovir, gemcitabine, idarubicin, ifosfamide, irinotecan, methotrexate, paclitaxel, pemetrexed, raltitrexed, topotecan and vincristine. Best conditions were tested with real samples from a hospital pharmacy chemotherapy compounding unit.
Polyester swabs (TX714 and TX716) gave satisfactory results for the desorption step for all compounds with mean recoveries of 90% and 95%, respectively. For the wiping step, higher recoveries were obtained using TX716 and isopropanol 75% as wiping solution. As anticipated, most intense contaminations were found close to the chemotherapy production site, on surfaces the most frequently in contact with operators' hands.
Wipe sampling method was successfully developed and applied to real samples to determine surface contamination with 23 antineoplastic agents in trace amounts.
优化擦拭采样程序，以评估医院药房中使用的 23 种抗肿瘤药物的表面污染情况。
使用经过验证的液相色谱-质谱（LC-MS/MS）方法评估各种参数（即采样设备、采样溶液、解吸模式）的影响，该方法能够定量分析广泛应用于医院药房的 23 种抗肿瘤药物：5-氟尿嘧啶、白消安、环磷酰胺、阿糖胞苷、达卡巴嗪、柔红霉素、多西他赛、多柔比星、表柔比星、依托泊苷、依托泊苷磷酸、氟达拉滨磷酸、更昔洛韦、吉西他滨、伊达比星、异环磷酰胺、伊立替康、甲氨蝶呤、紫杉醇、培美曲塞、雷替曲塞、拓扑替康和长春新碱。使用来自医院药房化疗配制单元的实际样品测试最佳条件。
聚酯拭子（TX714 和 TX716）对所有化合物的解吸步骤均能获得满意的结果，平均回收率分别为 90%和 95%。对于擦拭步骤，使用 TX716 和 75%异丙醇作为擦拭溶液可获得更高的回收率。正如预期的那样，在靠近化疗生产地点的地方，在操作人员手最常接触的表面上，发现了最强烈的污染。
成功开发并应用擦拭采样方法，以痕量测定 23 种抗肿瘤药物在表面的污染情况。
