Research Group of Membrane Molecular Interactions, School of Chemical and Food Engineering, Post-graduation Program in Technological and Environmental Chemistry, Federal University of Rio Grande, Av. Itália, km 8, Campus Carreiros, Rio Grande-RS 96203-900, Brazil.
Bioorg Chem. 2013 Dec;51:8-15. doi: 10.1016/j.bioorg.2013.08.004. Epub 2013 Sep 6.
In this study, the effect of α-eleostearic acid (α-ESA) on the lipid peroxidation of soybean asolectin (ASO) liposomes was investigated. This effect was correlated to changes caused by the fatty acid in the membrane dynamics. The influence of α-ESA on the dynamic properties of liposomes, such as hydration, mobility and order, were followed by horizontal attenuated total reflection Fourier transform infrared spectroscopy (HATR-FTIR), nuclear magnetic resonance (NMR), differential scanning calorimetry (DSC) and UV-vis techniques. The α-ESA showed an in vitro antioxidant activity against the damage induced by hydroxyl radical (OH) in ASO liposomes. The analysis of HATR-FTIR frequency shifts and bandwidths and (1)H NMR spin-lattice relaxation times, related to specific lipid groups, showed that α-ESA causes an ordering effect on the polar and interfacial regions of ASO liposomes, which may restrict the OH diffusion in the membrane. The DSC enthalpy variation analysis suggested that the fatty acid promoted a disordering effect on lipid hydrophobic regions, which may facilitate interactions between the reactive specie and α-ESA. Turbidity results showed that α-ESA induces a global disordering effect on ASO liposomes, which may be attributed to a change in the lipid geometry and shape. Results of this study may allow a more complete view of α-ESA antioxidant mode of action against OH, considering its influence on the membrane dynamics.
在这项研究中，研究了α-桐酸（α-ESA）对大豆溶血磷脂（ASO）脂质体脂质过氧化的影响。这种作用与脂肪酸引起的膜动力学变化有关。通过水平衰减全反射傅里叶变换红外光谱（HATR-FTIR）、核磁共振（NMR）、差示扫描量热法（DSC）和紫外-可见技术研究了α-ESA 对脂质体水合、流动性和有序性等动态特性的影响。α-ESA 对 ASO 脂质体中羟基自由基（OH）诱导的损伤表现出体外抗氧化活性。HATR-FTIR 频率位移和带宽以及与特定脂质基团相关的（1）H NMR 自旋晶格弛豫时间的分析表明，α-ESA 对 ASO 脂质体的极性和界面区域产生有序作用，这可能限制 OH 在膜中的扩散。DSC 焓变分析表明，脂肪酸对脂质疏水区产生了无序作用，这可能促进了反应物质与 α-ESA 之间的相互作用。浊度结果表明，α-ESA 诱导 ASO 脂质体整体无序化，这可能归因于脂质几何形状和形状的变化。考虑到α-ESA 对膜动力学的影响，这项研究的结果可能使我们更全面地了解其对 OH 的抗氧化作用机制。
